Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Guillaume, Lenoir"'
Autor:
Poul Nissen, Joseph A. Lyons, Cédric Montigny, Guillaume Lenoir, Thibaud Dieudonné, Milena Timcenko, Thomas Boesen
Publikováno v:
Timcenko, M, Dieudonné, T, Montigny, C, Boesen, T, Lyons, J A, Lenoir, G & Nissen, P 2021, ' Structural Basis of Substrate-Independent Phosphorylation in a P4-ATPase Lipid Flippase ', Journal of Molecular Biology, vol. 433, no. 16, 167062 . https://doi.org/10.1016/j.jmb.2021.167062
P4-ATPases define a eukaryotic subfamily of the P-type ATPases, and are responsible for the transverse flip of specific lipids from the extracellular or luminal leaflet to the cytosolic leaflet of cell membranes. The enzymatic cycle of P-type ATPases
Publikováno v:
Lyons, J A, Timcenko, M, Dieudonné, T, Lenoir, G & Nissen, P 2020, ' P4-ATPases : how an old dog learnt new tricks — structure and mechanism of lipid flippases ', Current Opinion in Structural Biology, vol. 63, pp. 65-73 . https://doi.org/10.1016/j.sbi.2020.04.001
Type 4 P-type ATPases (P4-ATPases) are lipid flippases that drive the active, inward directed translocation (flip) of lipids in eukaryotic membranes. The resulting lipid asymmetry potentiates the membrane and is essential for a wide range of cellular
Publikováno v:
BBA-Biochimica et Biophysica Acta
BBA-Biochimica et Biophysica Acta, Elsevier, 2016, pp.767-83. 〈10.1016/j.bbalip.2015.12.020〉
BBA-Biochimica et Biophysica Acta, Elsevier, 2016, pp.767-83. ⟨10.1016/j.bbalip.2015.12.020⟩
Montigny, C, Lyons, J, Champeil, P, Nissen, P & Lenoir, G 2016, ' On the molecular mechanism of flippase-and scramblase-mediated phospholipid transport ', B B A-Molecular and Cell Biology of Lipids, vol. 1861, no. 8, Part B . https://doi.org/10.1016/j.bbalip.2015.12.020
BBA-Biochimica et Biophysica Acta, 2016, pp.767-83. ⟨10.1016/j.bbalip.2015.12.020⟩
BBA-Biochimica et Biophysica Acta, Elsevier, 2016, pp.767-83. 〈10.1016/j.bbalip.2015.12.020〉
BBA-Biochimica et Biophysica Acta, Elsevier, 2016, pp.767-83. ⟨10.1016/j.bbalip.2015.12.020⟩
Montigny, C, Lyons, J, Champeil, P, Nissen, P & Lenoir, G 2016, ' On the molecular mechanism of flippase-and scramblase-mediated phospholipid transport ', B B A-Molecular and Cell Biology of Lipids, vol. 1861, no. 8, Part B . https://doi.org/10.1016/j.bbalip.2015.12.020
BBA-Biochimica et Biophysica Acta, 2016, pp.767-83. ⟨10.1016/j.bbalip.2015.12.020⟩
International audience; Phospholipid flippases are key regulators of transbilayer lipid asymmetry in eukaryotic cell membranes, critical to many trafficking and signaling pathways. P4-ATPases, in particular, are responsible for the uphill transport o
Autor:
Philippe Champeil, Pierre Le Maréchal, Joseph A. Lyons, Poul Nissen, José Luis Vázquez-Ibar, Cédric Montigny, Aurore Jacquot, Thibaud Dieudonné, Jakob Ulstrup, Miriam-Rose Ash, Guillaume Lenoir, Hassina Azouaoui
Publikováno v:
Journal of Biological Chemistry
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2017, 292 (19), pp.7954-7970. ⟨10.1074/jbc.M116.751487⟩
Journal of Biological Chemistry, 2017, 292 (19), pp.7954-7970. ⟨10.1074/jbc.M116.751487⟩
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2017, 292 (19), pp.7954-7970. 〈10.1074/jbc.M116.751487〉
Azouaoui, H, Montigny, C, Dieudonné, T, Champeil, P, Jacquot, A, Vázquez-Ibar, J L, le Maréchal, P, Ulstrup, J, Ash, M-R, Lyons, J A, Nissen, P & Lenoir, G 2017, ' A High and Phosphatidylinositol-4-phosphate (PI4P)-dependent ATPase Activity for the Drs2p/Cdc50p Flippase after Removal of its N-and C-terminal Extensions ', Journal of Biological Chemistry, vol. 292, pp. 7954-7970 . https://doi.org/10.1074/jbc.M116.751487
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2017, 292 (19), pp.7954-7970. ⟨10.1074/jbc.M116.751487⟩
Journal of Biological Chemistry, 2017, 292 (19), pp.7954-7970. ⟨10.1074/jbc.M116.751487⟩
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2017, 292 (19), pp.7954-7970. 〈10.1074/jbc.M116.751487〉
Azouaoui, H, Montigny, C, Dieudonné, T, Champeil, P, Jacquot, A, Vázquez-Ibar, J L, le Maréchal, P, Ulstrup, J, Ash, M-R, Lyons, J A, Nissen, P & Lenoir, G 2017, ' A High and Phosphatidylinositol-4-phosphate (PI4P)-dependent ATPase Activity for the Drs2p/Cdc50p Flippase after Removal of its N-and C-terminal Extensions ', Journal of Biological Chemistry, vol. 292, pp. 7954-7970 . https://doi.org/10.1074/jbc.M116.751487
International audience; P4-ATPases, also known as phospholipid flippases, are responsible for creating and maintaining transbilayer lipid asymmetry in eukaryotic cell membranes. Here, we use limited proteolysis to investigate the role of the N and C
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78a13bfa67cbd26904683eacf27edde2
https://hal.archives-ouvertes.fr/hal-01697714
https://hal.archives-ouvertes.fr/hal-01697714
Autor:
Jesper V. Møller, Marc le Maire, Simon Babin, Stéphane Orlowski, Sten Lund, Philippe Champeil, Guillaume Lenoir, Cédric Montigny
Publikováno v:
Analytical Biochemistry
Analytical Biochemistry, Elsevier Masson, 2016, 511, pp.31-35
Champeil, P, Orlowski, S, Babin, S, Lund, S, le Maire, M, Møller, J, Lenoir, G & Montigny, C 2016, ' A robust method to screen detergents for membrane protein stabilization, revisited ', Analytical Biochemistry, vol. 511, pp. 31-5 . https://doi.org/10.1016/j.ab.2016.07.017
Analytical Biochemistry, 2016, 511, pp.31-35
Analytical Biochemistry, Elsevier Masson, 2016, 511, pp.31-35
Champeil, P, Orlowski, S, Babin, S, Lund, S, le Maire, M, Møller, J, Lenoir, G & Montigny, C 2016, ' A robust method to screen detergents for membrane protein stabilization, revisited ', Analytical Biochemistry, vol. 511, pp. 31-5 . https://doi.org/10.1016/j.ab.2016.07.017
Analytical Biochemistry, 2016, 511, pp.31-35
International audience; This report is a follow up of our previous paper (Lund, Orlowski, de Foresta, Champeil, le Maire and Møller (1989), J Biol Chem 264:4907–4915) showing that solubilization in detergent of a membrane protein may interfere wit
Autor:
Paulette Decottignies, Philippe Champeil, Manuel Garrigos, Frank Fijalkowski, Poul Nissen, Guillaume Lenoir, Rosa L. López-Marqués, Hassina Azouaoui, Pontus Gourdon, Miriam-Rose Ash, Christina Grønberg, Cédric Montigny, Michael G. Palmgren, Aurore Jacquot, Marc le Maire
Publikováno v:
PLoS ONE
Azouaoui, H, Montigny, C, Ash, M-R, Fijalkowski, F A, Jacquot, A, Grønberg, C, Lopez Marques, R L, Palmgren, M B, Garrigos, M, le Maire, M, Decottignies, P, Gourdon, P E, Nissen, P, Champeil, P & Lenoir, G 2014, ' A high-yield co-expression system for the purification of an intact drs2p-cdc50p lipid flippase complex, critically dependent on and stabilized by phosphatidylinositol-4-phosphate ', PloS one, vol. 9, no. 11, e112176, pp. 1-16 . https://doi.org/10.1371/journal.pone.0112176
PLoS ONE, 2014, 9 (11), pp.e112176. ⟨10.1371/journal.pone.0112176⟩
Azouaoui, H, Montigny, C, Ash, M-R, Fijalkowski, F, Jacquot, A, Grønberg, C, López-Marqués, R L, Palmgren, M G, Garrigos, M, le Maire, M, Decottignies, P, Gourdon, P, Nissen, P, Champeil, P & Lenoir, G 2014, ' A high-yield co-expression system for the purification of an intact drs2p-cdc50p lipid flippase complex, critically dependent on and stabilized by phosphatidylinositol-4-phosphate ', PLOS ONE, vol. 9, no. 11, e112176 . https://doi.org/10.1371/journal.pone.0112176
PLoS ONE, Public Library of Science, 2014, 9 (11), pp.e112176. ⟨10.1371/journal.pone.0112176⟩
PLoS ONE, Vol 9, Iss 11, p e112176 (2014)
Azouaoui, H, Montigny, C, Ash, M-R, Fijalkowski, F A, Jacquot, A, Grønberg, C, Lopez Marques, R L, Palmgren, M B, Garrigos, M, le Maire, M, Decottignies, P, Gourdon, P E, Nissen, P, Champeil, P & Lenoir, G 2014, ' A high-yield co-expression system for the purification of an intact drs2p-cdc50p lipid flippase complex, critically dependent on and stabilized by phosphatidylinositol-4-phosphate ', PloS one, vol. 9, no. 11, e112176, pp. 1-16 . https://doi.org/10.1371/journal.pone.0112176
PLoS ONE, 2014, 9 (11), pp.e112176. ⟨10.1371/journal.pone.0112176⟩
Azouaoui, H, Montigny, C, Ash, M-R, Fijalkowski, F, Jacquot, A, Grønberg, C, López-Marqués, R L, Palmgren, M G, Garrigos, M, le Maire, M, Decottignies, P, Gourdon, P, Nissen, P, Champeil, P & Lenoir, G 2014, ' A high-yield co-expression system for the purification of an intact drs2p-cdc50p lipid flippase complex, critically dependent on and stabilized by phosphatidylinositol-4-phosphate ', PLOS ONE, vol. 9, no. 11, e112176 . https://doi.org/10.1371/journal.pone.0112176
PLoS ONE, Public Library of Science, 2014, 9 (11), pp.e112176. ⟨10.1371/journal.pone.0112176⟩
PLoS ONE, Vol 9, Iss 11, p e112176 (2014)
International audience; P-type ATPases from the P4 subfamily (P4-ATPases) are energy-dependent transporters, which are thought to establish lipid asymmetry in eukaryotic cell membranes. Together with their Cdc50 accessory subunits, P4-ATPases couple
Autor:
Philippe Champeil, Jesper V. Møller, Martin Picard, Pierre Falson, Guillaume Lenoir, Marc le Maire
Publikováno v:
Journal of Biological Chemistry
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2004, 279 (31), pp.32125-32133. ⟨10.1074/jbc.M402934200⟩
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2004, 279, pp.32125-32133
Journal of Biological Chemistry, 2004, 279 (31), pp.32125-32133. ⟨10.1074/jbc.M402934200⟩
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2004, 279 (31), pp.32125-32133. ⟨10.1074/jbc.M402934200⟩
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2004, 279, pp.32125-32133
Journal of Biological Chemistry, 2004, 279 (31), pp.32125-32133. ⟨10.1074/jbc.M402934200⟩
International audience; Wild-type (WT) and the double mutant D813A,D818A (ADA) of the L6-7 loop of SERCA1a were expressed in yeast, purified, and reconstituted into lipids. This allowed us to functionally study these ATPases by both kinetic and spect
Autor:
Jesper V. Møller, Christophe H. Marchand, Cédric Montigny, Marc le Maire, Philippe Champeil, B Juul, Chikashi Toyoshima, Guillaume Lenoir
Publikováno v:
Journal of Biological Chemistry. 277:38647-38659
After treatment of sarcoplasmic reticulum Ca(2+)-ATPase with proteinase K (PK) in the presence of Ca(2+) and a protecting non-phosphorylated ligand (e.g. adenosine 5'-(beta,gamma-methylenetriphosphate), we were able to prepare in high yield an ATPase
Autor:
Guillaume Lenoir, Aurore Jacquot, Raphaëlle Barry, Hanka Hennrich, Philippe Champeil, Marc le Maire, Joost C. M. Holthuis, Christine Jaxel, Cédric Montigny
Here, Drs2p, a yeast lipid translocase that belongs to the family of P(4)-type ATPases, was overexpressed in the yeast Saccharomyces cerevisiae together with Cdc50p, its glycosylated partner, as a result of the design of a novel co-expression vector.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b969913879517963c37c01af3c483a0
https://europepmc.org/articles/PMC3531771/
https://europepmc.org/articles/PMC3531771/
Autor:
Cédric Montigny, Marc le Maire, Denyse Thinès, Fabienne Corre, Pierre Falson, Thierry Menguy, Per Amstrup Pedersen, Guillaume Lenoir
Publikováno v:
University of Copenhagen
Annals of the New York Academy of Sciences
Annals of the New York Academy of Sciences, Wiley, 2003, 986, pp.312-314
Annals of the New York Academy of Sciences
Annals of the New York Academy of Sciences, Wiley, 2003, 986, pp.312-314
Much effort has been invested to express the SERCA1a Ca2+-ATPase, a large membrane protein that transports Ca2+ across the sarcoplasmic reticulum membrane. Various expression systems have been set up with that goal, including COS cells1,2 and Spodopt