Zobrazeno 1 - 10
of 27
pro vyhledávání: '"Amy E. Herr"'
Autor:
James Eberwine, Junhyong Kim, Ron C. Anafi, Steven Brem, Maja Bucan, Stephen A. Fisher, M. Sean Grady, Amy E. Herr, David Issadore, Hyejoong Jeong, HyunBum Kim, Daeyeon Lee, Stanislav Rubakhin, Jai-Yoon Sul, Jonathan V. Sweedler, John A. Wolf, Kenneth S. Zaret, James Zou
Publikováno v:
Nat Methods
We argue that the study of single-cell subcellular organelle omics is needed to understand and regulate cell function. This requires and is being enabled by new technology development.
Publikováno v:
SLAS Technol
New pipelines are required to automate the quantitation of emerging high-throughput electrophoretic (EP) assessment of DNA damage, or proteoform expression in single cells. EP cytometry consists of thousands of Western blots performed on a microscope
Autor:
Kristin E. Burnum-Johnson, Thomas P. Conrads, Richard R. Drake, Amy E. Herr, Ravi Iyengar, Ryan T. Kelly, Emma Lundberg, Michael J. MacCoss, Alexandra Naba, Garry P. Nolan, Pavel A. Pevzner, Karin D. Rodland, Salvatore Sechi, Nikolai Slavov, Jeffrey M. Spraggins, Jennifer E. Van Eyk, Marc Vidal, Christine Vogel, David R. Walt, Neil L. Kelleher
All human diseases involve proteins, yet our current tools to characterize and quantify them are limited. To better elucidate proteins across space, time, and molecular composition, we provide a >10 years of projection for technologies to meet the ch
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2fb2acd4107948a012703b294d143bbe
Publikováno v:
PLoS ONE
PLoS ONE, Vol 16, Iss 7, p e0254783 (2021)
PLoS ONE, Vol 16, Iss 7, p e0254783 (2021)
An array of isoforms of the nuclear estrogen receptor alpha (ER-α) protein contribute to heterogeneous response in breast cancer (BCa); yet, a single-cell analysis tool that distinguishes the full-length ER-α66 protein from the activation function-
Publikováno v:
Angewandte Chemie (International ed. in English), vol 55, iss 40
To measure protein isoforms in individual mammalian cells, we report single-cell resolution isoelectric focusing (scIEF) and high-selectivity immunoprobing. Microfluidic design and photoactivatable materials establish the tunable pH gradients require
Publikováno v:
Angew Chem Int Ed Engl
Integrating 2D culture of adherent mammalian cells with single-cell western blotting (in situ scWB) uses microfluidic design to eliminate the requirement for trypsin release of cells to suspension, prior to single-cell isolation and protein analysis.
Autor:
Kevin A. Yamauchi, Todd A. Duncombe, Julea Vlassakis, Chi-Chih Kang, Elly Sinkala, Amy E. Herr
Publikováno v:
Nature Protocols. 11:1508-1530
This protocol describes how to perform western blotting on individual cells to measure cell-to-cell variation in protein expression levels and protein state. like conventional western blotting, single-cell western blotting (scWB) is particularly usef
Autor:
Toby M. Ward, Niren Murthy, Todd A. Duncombe, Santanu Maity, Mark D. Pegram, Amy E. Herr, Chi-Chih Kang
Publikováno v:
Duncombe, TA; Kang, CC; Maity, S; Ward, TM; Pegram, MD; Murthy, N; et al.(2016). Hydrogel Pore-Size Modulation for Enhanced Single-Cell Western Blotting. Advanced Materials, 28(2), 327-334. doi: 10.1002/adma.201503939. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/99c7h5dg
Advanced materials (Deerfield Beach, Fla.), vol 28, iss 2
Advanced materials (Deerfield Beach, Fla.), vol 28, iss 2
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Pore-gradient microgel arrays enable thousands of parallel high-resolution single-cell protein electrophoresis separations for targets accross a wide molecular mass (25-289 kDa), yet within 1 mm sep
Autor:
Amy E. Herr
Publikováno v:
The FASEB Journal. 32
Autor:
Chi-Chih Kang, Courtney Schiffman, Haiyan Huang, Toby M. Ward, Amy E. Herr, Mark D. Pegram, Jessica Bockhorn
Publikováno v:
npj Precision Oncology, Vol 2, Iss 1, Pp 1-10 (2018)
NPJ Precision Oncology
NPJ Precision Oncology
In addition to canonical oncoproteins, truncated isoforms and proteolysis products are implicated in both drug resistance and disease progression. In HER2-positive breast tumors, expression of truncated HER2 isoforms resulting from alternative transl