Zobrazeno 1 - 10
of 30
pro vyhledávání: '"Nigel, M Hooper"'
Publikováno v:
Journal of Cell Science. 117:5079-5086
The glycosyl-phosphatidylinositol (GPI) anchor mediates the apical sorting of proteins in polarised epithelial cells through its interaction with lipid rafts. Here we investigated the signals required for the apical targeting of the naturally N-glyco
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Biomembranes. 1511(2):317-329
The 1I gene is expressed in the prespore cells of culminating Dictyostelium discoideum. The open reading frame of 1I cDNA encodes a protein of 155 amino acids with hydrophobic segments at both its NH2- and COOH-termini that are indicative of a glycos
Autor:
Sara Movahedi, Nigel M. Hooper
Publikováno v:
Biochemical Journal. 326:531-537
Membrane dipeptidase (MDP; EC 3.4.13.19) enzymic activity that was inhibited by cilastatin has been detected on the surface of 3T3-L1 cells. On differentiation of the cells from fibroblasts to adipocytes the activity of MDP increased 12-fold. Immunoe
Autor:
Angela Mehlert, Anthony J. Turner, Michael A. J. Ferguson, Nigel M. Hooper, Ian Andrew Brewis
Publikováno v:
Journal of Biological Chemistry. 270:22946-22956
The glycan core structures of the glycosyl-phosphatidylinositol (GPI) anchors on porcine and human renal membrane dipeptidase (EC 3.4.13.19) were determined following deamination and reduction by a combination of liquid chromatography, exoglycosidase
Publikováno v:
Biochemical Journal. 303:633-638
Incubation of pig kidney microvillar membranes with Bacillus thuringiensis or Staphylococcus aureus phosphatidylinositol-specific phospholipase C (PI-PLC) resulted in the release of a number of glycosyl-phosphatidylinositol (GPI)-anchored hydrolases,
Publikováno v:
FEBS Letters. 349:50-54
Pig renal membrane dipeptidase cDNA has been expressed in COS-1 cells. Directed mutagenesis was used to investigate the roles of some conserved histidyl and aspartyl residues. Mutation of His219 to Arg, Lys or Leu results in complete abolition of enz
Autor:
Nigel M. Hooper, Stephen Tieku
Publikováno v:
Biochemical Pharmacology
The effects of a range of metallopeptidase inhibitors on the activities of the porcine kidney cell surface zinc aminopeptidases, aminopeptidase A (AP-A; EC 3.4.11.2), aminopeptidase N (AP-N; EC 3.4.11.7) and aminopeptidase W (AP-W; EC 3.4.11.16), hav
Publikováno v:
Biochemical Journal. 265:429-433
Renal dipeptidase (EC 3.4.13.11) has been purified from human kidney cortex by affinity chromatography on cilastatin-Sepharose following solubilization with either n-octyl-beta-D-glucopyranoside or bacterial phosphatidylinositol-specific phospholipas
Publikováno v:
The FASEB Journal. 21