Zobrazeno 1 - 10
of 50
pro vyhledávání: '"Uwe T. Bornscheuer"'
Autor:
Matúš Gajdoš, Mahsa Takhtehchian, Uwe T. Bornscheuer, Robert Kourist, Alexia Kindler, Ruth Birner-Gruenberger, Barbara Darnhofer, Ivana Drienovská, Mark Dörr
Publikováno v:
Chemistry (Weinheim an Der Bergstrasse, Germany)
Drienovská, I, Gajdoš, M, Kindler, A, Takhtehchian, M, Darnhofer, B, Birner-Gruenberger, R, Dörr, M, Bornscheuer, U T & Kourist, R 2020, ' Folding Assessment of Incorporation of Noncanonical Amino Acids Facilitates Expansion of Functional-Group Diversity for Enzyme Engineering ', Chemistry-A European Journal, vol. 26, no. 54, pp. 12338-12342 . https://doi.org/10.1002/chem.202002077
Chemistry-A European Journal, 26(54), 12338-12342. Wiley-VCH Verlag
Drienovská, I, Gajdoš, M, Kindler, A, Takhtehchian, M, Darnhofer, B, Birner-Gruenberger, R, Dörr, M, Bornscheuer, U T & Kourist, R 2020, ' Folding Assessment of Incorporation of Noncanonical Amino Acids Facilitates Expansion of Functional-Group Diversity for Enzyme Engineering ', Chemistry-A European Journal, vol. 26, no. 54, pp. 12338-12342 . https://doi.org/10.1002/chem.202002077
Chemistry-A European Journal, 26(54), 12338-12342. Wiley-VCH Verlag
Protein design is limited by the diversity of functional groups provided by the canonical protein „building blocks“. Incorporating noncanonical amino acids (ncAAs) into enzymes enables a dramatic expansion of their catalytic features. For this, q
Publikováno v:
Bioorganicmedicinal chemistry. 43
Apremilast is an important active pharmaceutical ingredient that relies on a resolution to produce the key chiral amine intermediate. To provide a new catalytic and enzymatic process for Apremilast, we performed the directed evolution of the amine tr
Publikováno v:
Monatshefte Fur Chemie
This study investigates the substrate profile of cycloalkanone monooxygenase and 2-oxo-Δ3-4,5,5-trimethylcyclopentenylacetyl-coenzyme A monooxygenase, two recently discovered enzymes of the Baeyer–Villiger monooxygenase family, used as whole-cell
Publikováno v:
ChemCatChem. 5:3529-3532
Publikováno v:
Journal of Molecular Catalysis B: Enzymatic. 74:144-150
Glyceraldehyde and glyceric acid are both valuable chiral starting materials. Aldehyde dehydrogenases (ALDHs) accept a broad scope of endo- and exogenous aldehydes, such as glyceraldehyde, and convert them into the corresponding carboxylic acid. Here
Publikováno v:
Applied Microbiology and Biotechnology. 93:1119-1126
An esterase from Pseudomonas putida JD1 (PPE) was successfully cloned, actively expressed in Escherichia coli, and characterized. It was discovered that PPE is more active towards short-chain esters, hydrolyzed δ-valerolactone, and e-caprolactone an
Publikováno v:
Journal of Molecular Catalysis B: Enzymatic. 70:88-94
Six esterases isolated from sequence and structure-guided genome mining approaches were evaluated for the kinetic resolution of secondary and tertiary alcohols that find application in the fine chemical and pharmaceutical industries. Activity and ena
Autor:
Robert Kourist, Uwe T. Bornscheuer, Pilar Diaz, Arnau Bassegoda, Giang-Son Nguyen, Marlen Schmidt
Publikováno v:
ChemCatChem. 2:962-967
Protein engineering is a very powerful tool to optimize en- zymes for specific applications and thus provide important chiral building blocks such as tertiary alcohols. By use of struc- tural comparisons, esterase from Paenibacillus barcinonensis (Es
Publikováno v:
Biocatalysis and Biotransformation. 28:201-208
The GGG(A)X motif has been reported to determine the activity of esterases or lipases towards tertiary alcohols. The γ-isoenzyme of pig liver esterase (γ-PLE, PLE 1) contains a GGGX motif; this motif was systematically mutated to the corresponding
Autor:
Isabel W. C. E. Arends, Sebastian Bartsch, Jarle Holt, Adriaan J. Minnaard, Michiel Wiggers, Robert Kourist, Ulf Hanefeld, Uwe T. Bornscheuer
Publikováno v:
Journal of Molecular Catalysis B: Enzymatic, 60(1-2), 82-86. ELSEVIER SCIENCE BV
The activity and enantioselectivity of several mutants of the esterase BS2 from Bacillus subtilis have been investigated. In the enzymatic hydrolysis of alpha,alpha-disubstituted cyanohydrin acetates, a class of tert. alcohol esters, they were active