Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Tadazumi Komiyama"'
Autor:
M. Enamul Kabir, Tadazumi Komiyama, Masahiko Miyamoto, Mahbubur Rahman, Yasuhiro Furuichi, Senthilkumar Krishnaswamy
Publikováno v:
Journal of Molecular Recognition. 24:631-641
Existing antifungal drugs are notable for their inability to act rapidly, as well as their toxicity and limited spectrum. The identification of fungal-specific genes and virulence factors would provide targets for new and influential drugs. The displ
Autor:
Masahiko Miyamoto, Tadazumi Komiyama, Yasuhiro Furuichi, M. Enamul Kabir, Mahbubur Rahman, Senthilkumar Krishnaswamy
Publikováno v:
Journal of Immunological Methods. 366:60-68
Aspergillus fumigatus causes the highly lethal form of invasive aspergillosis (IA). In the present study to develop a novel anti-fungal drug for protection against invasive disease, we identified a single chain fragment variable (scFv) antibody (scFv
Publikováno v:
Yeast. 28:27-41
We screened a set of Saccharomyces cerevisiae deletion mutants for resistance to killer toxin HM-1, which kills susceptible yeasts through inhibiting 1,3-beta-glucan synthase. By using HM-1 plate assay, we found that eight gene-deletion mutants had h
Autor:
Dakshnamurthy Selvakumar, Masahiko Miyamoto, Tetsuya Kimura, Yasuhiro Furuichi, Tadazumi Komiyama, Naohiko Onozato
Publikováno v:
Microbiology. 152:2951-2958
Diethylpyrocarbonate modification and site-directed mutagenesis studies of histidine-35 in HM-1 killer toxin (HM-1) have shown that a specific feature, the imidazole side chain of histidine-35, is essential for the expression of the killing activity.
Publikováno v:
The Journal of Antibiotics. 59:73-79
Recombinant single-chain fragment variable (scFv) anti-idiotypic antibodies were produced to represent the internal image of a HM-1 killer toxin, which is characterized by a wide spectrum of anti-fungal activity through inhibiting beta-1,3-glucan syn
Publikováno v:
Journal of Biochemistry. 137:517-522
Each of the aromatic, acidic and basic amino acid residues in HM-1 were separately substituted with alanine by site-directed mutagenesis. The mutant genes were successfully expressed in HM-1 resistant Saccharomyces cerevisiae. HM-1 gene analogues cor
Publikováno v:
Biological and Pharmaceutical Bulletin. 25:959-965
The effects of HM-1 killer toxin (HM-1) on yeast spheroplasts of Saccharomyces cerevisiae were examined under osmotically stabilized conditions. Prolonged incubation of spheroplasts in nutrient-rich media resulted in an increase in volume, accompanie
Autor:
Takahide Watanabe, Hiroshi Urakami, Tatsuo Ohta, Tsuyoshi Takasuka, Yasuhiro Furuichi, Tadazumi Komiyama, Misako Satoh, Yasuhiko Shiratori
Publikováno v:
Journal of Biochemistry. 119:731-736
The cytocidal effect of HM-1 produced by Hansenula mrakii on yeast Saccharomyces cerevisiae cells was studied. The HM-1 strongly inhibited the growth of S. cerevisiae cells at a low concentration (IC50: 2.1 x 10(-8) M) by reducing the number of viabl
Publikováno v:
Yeast (Chichester, England). 29(11)
Fps1p is an aquaglyceroporin important for turgor regulation of Saccharomyces cerevisiae. Previously we reported the involvement of Fps1p in the yeast-killing action of killer toxin HM-1. The fps1 cells showed a high HM-1-resistant phenotype in hypot
Autor:
Senthilkumar Krishnaswamy, Masahiko Miyamoto, Dakshnamurthy Selvakumar, M. Enamul Kabir, Nurul Karim, Yasuhiro Furuichi, Tadazumi Komiyama
Publikováno v:
Applied microbiology and biotechnology. 92(6)
Based on anti-idiotypic network theory in light of the need for new antifungal drugs, we attempted to identify biologically active fragments from HM-1 yeast killer toxin and its anti-idiotypic antibody and to compare their potency as an antifungal ag