Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Anders, Robert A."'
Autor:
Per Balschmidt, Ivan Diers, Svend Ludvigsen, Anders Robert Sorensen, Thomas Kjeldsen, Niels C. Kaarsholm
Publikováno v:
Journal of Biological Chemistry. 277:18245-18248
Adaptation to efficient heterologous expression is a prerequisite for recombinant proteins to fulfill their clinical and biotechnological potential. We describe a rational strategy to optimize the secretion efficiency in yeast of an insulin precursor
Autor:
Thomas Trüb, Ib Jonassen, Lauge Schäffer, Anders Robert Sorensen, Claus Kristensen, Christoph Schmid, Peter Kurtzhals
Publikováno v:
Scopus-Elsevier
In recent years, analogs of human insulin have been engineered with the aim of improving therapy for people with diabetes. To ensure that the safety profile of the human hormone is not compromised by the molecular modifications, the toxico-pharmacolo
Publikováno v:
Journal of Pharmaceutical Sciences. 84:1164-1168
The insulin hexamer has two high-affinity metal ion binding sites, each involving three HisB10 residues, one from each dimer. Insulin hexamers containing Co2+ at both these sites were oxidized to form a stable Co(3+)-insulin complex. It is shown that
Autor:
Carsten Enggaard Stidsen, Sara G. Vienberg, Stephan D. Bouman, Anders Robert Sorensen, Thomas Kjeldsen, Erica Nishimura, Tine Glendorf, Birgitte Andersen, Heidi Sørensen, Grith Skytte Olsen
Publikováno v:
The Biochemical journal. 440(3)
The relative expression patterns of the two IR (insulin receptor) isoforms, +/− exon 11 (IR-B/IR-A respectively), are tissue-dependent. Therefore we have developed insulin analogues with different binding affinities for the two isoforms to test whe
Autor:
Tine Glendorf, Thomas Kjeldsen, Anders Robert Sorensen, Erica Nishimura, Mathias Norrman, Carsten Enggaard Stidsen
Publikováno v:
PLoS ONE
PLoS ONE, Vol 6, Iss 5, p e20288 (2011)
PLoS ONE, Vol 6, Iss 5, p e20288 (2011)
BACKGROUND: The insulin receptor (IR) exists in two isoforms, A and B, and the isoform expression pattern is tissue-specific. The C-terminus of the insulin B chain is important for receptor binding and has been shown to contact the IR just adjacent t
Autor:
Erica Nishimura, Ib Jonassen, Carsten Enggaard Stidsen, Anders Robert Sorensen, Jeppe Sturis, Ulla Ribel, Stephan D. Bouman, Christian L. Brand, Peter Kurtzhals
Publikováno v:
Diabetes, obesitymetabolism. 12(8)
Aim: To compare the properties of insulin detemir with human insulin or insulin aspart in various in vitro and in vivo experiments, thereby highlighting the importance of performing dose–response studies when investigating insulin analogues, in thi
Publikováno v:
Biochemistry. 47(16)
Conjointly, the solvent-exposed residues of the central alpha-helix of the B chain form a well-defined ridge, which is flanked and partly overlapped by the two described insulin receptor binding surfaces on either side of the insulin molecule. To eva
Autor:
Kirsten Drejer, Bo Falck Hansen, Finn C. Wiberg, Gillian M. Danielsen, Harald Klein, Anker G. Lundemose, Anders Robert Sorensen
The metabolic and mitogenic potencies of six different insulin analogues were determined by measuring glucose transport in primary adipocytes and DNA synthesis in CHO cells respectively. Three analogues showed a disproportionately high mitogenic pote
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d2a3b5c042b66cb7f3d16c6ef6daaa8
https://europepmc.org/articles/PMC1217182/
https://europepmc.org/articles/PMC1217182/
Autor:
Tine Glendorf, Thomas Kjeldsen, Carsten Enggaard Stidsen, Erica Nishimura, Bo Falck Hansen, Anne Charlotte Hegelund, Louise Knudsen, Anders Robert Sorensen
Publikováno v:
PLoS ONE, Vol 7, Iss 2, p e29198 (2012)
PLoS ONE
PLoS ONE
Background Insulin analogues comprising acidic amino acid substitutions at position B10 have previously been shown to display increased mitogenic potencies compared to human insulin and the underlying molecular mechanisms have been subject to much sc
Autor:
Kirsten Drejer, Aa. Vølund, J. Schlichtkrull, I. Jensen, Anders Robert Sorensen, J. Markussen, J. Brange, U. Ribel
Publikováno v:
Diabetic medicine : a journal of the British Diabetic Association. 8(9)
Analogues of human insulin designed to have improved absorption properties after subcutaneous injection have been prepared by recombinant DNA technology. Five rapidly absorbed analogues, being predominantly in mono- or di-meric states in the pharmace