Zobrazeno 1 - 8
of 8
pro vyhledávání: '"C. Shan Xu"'
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
Scientific Reports
Scientific Reports
Modern morphological and structural studies are coming to a new level by incorporating the latest methods of three-dimensional electron microscopy (3D-EM). One of the key problems for the wide usage of these methods is posed by difficulties with samp
Autor:
David Peale, Gleb Shtengel, David J. Solecki, Jennifer Lippincott-Schwartz, Daniel R. Stabley, Tom Kirchhausen, Harald F. Hess, Nirmala Iyer, Song Pang, David P. Hoffman, Kathy Schaefer, Melanie Freeman, Daniel E. Milkie, Lei Wang, Eric Betzig, H. Amalia Pasolli, Abbas Shirinifard, Chi-Lun Chang, Kirby R. Campbell, John A. Bogovic, Wim Pomp, C. Shan Xu
Publikováno v:
Science
Visualizing whole cells at many scales Cells need to compartmentalize thousands of distinct proteins, but the nanoscale spatial relationship of many proteins to overall intracellular ultrastructure remains poorly understood. Correlated light and elec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4020d4606b2e1a7dbdff319dc7424a2
Autor:
Kenneth J, Hayworth, David, Peale, Michał, Januszewski, Graham W, Knott, Zhiyuan, Lu, C Shan, Xu, Harald F, Hess
Publikováno v:
Nature methods. 17(1)
We demonstrate gas cluster ion beam scanning electron microscopy (SEM), in which wide-area ion milling is performed on a series of thick tissue sections. This three-dimensional electron microscopy technique acquires datasets with 10 nm isotropic reso
Autor:
Eric Betzig, Igor Pisarev, Srigokul Upadhyayula, Shu-Hsien Sheu, Yongxin Zhao, Austin R. Graves, Christopher T. Zugates, Sean G. Megason, John A. Bogovic, Harald F. Hess, Adam W. Hantman, Song Pang, C. Shan Xu, Susan Tappan, Kishore R. Mosaliganti, Yoshinori Aso, Ved P. Singh, Shoh Asano, Jennifer Lippincott-Schwartz, Carolyn Ott, Gerald M. Rubin, H. Amalia Pasolli, Jennifer Colonell, Edward S. Boyden, Daniel E. Milkie, Grace H. Huynh, Tom Kirchhausen, Stephan Saalfeld, Alfredo Rodriguez, Tsung-Li Liu, Ruixuan Gao
Publikováno v:
PMC
INTRODUCTION Neural circuits across the brain are composed of structures spanning seven orders of magnitude in size that are assembled from thousands of distinct protein types. Electron microscopy has imaged densely labeled brain tissue at nanometer-
Autor:
C Shan Xu, Kenneth J Hayworth, Zhiyuan Lu, Patricia Grob, Ahmed M Hassan, José G García-Cerdán, Krishna K Niyogi, Eva Nogales, Richard J Weinberg, Harald F Hess
Publikováno v:
Xu, CS; Hayworth, KJ; Lu, Z; Grob, P; Hassan, AM; García-Cerdán, JG; et al.(2017). Enhanced FIB-SEM systems for large-volume 3D imaging. eLife, 6. doi: 10.7554/eLife.25916. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/3sr9c8s2
eLife
eLife, Vol 6 (2017)
eLife
eLife, Vol 6 (2017)
Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) can automatically generate 3D images with superior z-axis resolution, yielding data that needs minimal image registration and related post-processing. Obstacles blocking wider adoption of FIB-SE
Autor:
Pietro De Camilli, C. Shan Xu, Harald F. Hess, Yumei Wu, Christina Whiteus, Kenneth J. Hayworth, Richard J. Weinberg
Close appositions between the membrane of the endoplasmic reticulum (ER) and other intracellular membranes have important functions in cell physiology. These include lipid homeostasis, regulation of Ca2+ dynamics, and control of organelle biogenesis
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8b3b54a1a702fdac7986ec38c0d46752
Publikováno v:
Proceedings of the National Academy of Sciences. 109:6136-6141
Microscopic images of specific proteins in their cellular context yield important insights into biological processes and cellular architecture. The advent of superresolution optical microscopy techniques provides the possibility to augment EM with na
Autor:
Juan Carlos Tapia, Jeff W. Lichtman, Graham Knott, Zhiyuan Lu, Kenneth J. Hayworth, Richard D. Fetter, C. Shan Xu, Harald F. Hess
Publikováno v:
Nature methods
Focused-ion-beam scanning electron microscopy (FIB-SEM) has become an essential tool for studying neural tissue at resolutions below 10 nm x 10 nm x 10 nm, producing data sets optimized for automatic connectome tracing. We present a technical advance