Zobrazeno 1 - 10
of 10
pro vyhledávání: '"James T. Koerber"'
Autor:
Kunal V. Gujraty, David V. Schaffer, James T. Koerber, Sridhar R. Bethi, Ravi S. Kane, Jae Hyung Jang
Publikováno v:
Gene therapy, vol 17, iss 11
Gene therapy
Gene therapy
Adeno-associated viral (AAV) vectors, which are undergoing broad exploration in clinical trials, have significant promise for therapeutic gene delivery because of their safety and delivery efficiency. Gene delivery technologies capable of mediating l
Publikováno v:
Nature Protocols. 1:701-706
Rational design of improved gene delivery vehicles is a challenging and potentially time-consuming process. As an alternative approach, directed evolution can provide a rapid and efficient means for identifying novel proteins with improved function.
Publikováno v:
Journal of molecular biology, vol 427, iss 2
Antibody phage display libraries combined with high-throughput selections have recently demonstrated tremendous promise to create the next generation of renewable, recombinant antibodies to study proteins and their many post-translational modificatio
Autor:
John G. Flannery, Deniz Dalkara, James T. Koerber, David V. Schaffer, Ryan R. Klimczak, Jae Hyung Jang
The natural tropism of most viral vectors, including adeno-associated viral (AAV) vectors, leads to predominant transduction of neurons and epithelia within the central nervous system (CNS) and retina. Despite the clinical relevance of glia for homeo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cf15ce73b4bdeed57e8780a853f85fb6
https://europepmc.org/articles/PMC2788045/
https://europepmc.org/articles/PMC2788045/
Autor:
Matthew J. Murtha, Shaf Keshavjee, James T. Koerber, Brian K. Kaspar, David D. Dickey, Joseph Zabner, Katherine J. D. A. Excoffon, David V. Schaffer
Respiratory viruses evolve to maintain infectivity levels that permit spread yet prevent host and virus extinction, resulting in surprisingly low infection rates. Respiratory viruses harnessed as gene therapy vectors have illustrated this limitation.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95f2a017409d37febfc86155f4e37354
https://europepmc.org/articles/PMC2646629/
https://europepmc.org/articles/PMC2646629/
Publikováno v:
Annual review of biomedical engineering. 10
Viruses can be engineered to efficiently deliver exogenous genes, but their natural gene delivery properties often fail to meet human therapeutic needs. Therefore, engineering viral vectors with new properties, including enhanced targeting abilities
Autor:
James T, Koerber, David V, Schaffer
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 434
The engineering of novel properties and functions into viral vectors for improved gene delivery remains a barrier to the development of efficient, customized gene delivery vehicles. Rational methods for designing improved viral vectors are often expe
Publikováno v:
Human gene therapy. 18(4)
Adeno-associated virus (AAV) is a promising vehicle for gene therapy, which will rely on the generation of high-titer, high-purity recombinant vectors. However, numerous purification protocols can involve challenging optimization or scalability issue
Publikováno v:
Nature biotechnology. 24(2)
Adeno-associated viral vectors are highly safe and efficient gene delivery vehicles. However, numerous challenges in vector design remain, including neutralizing antibody responses, tissue transport and infection of resistant cell types. Changes must
Publikováno v:
Pharmaceutical research. 21(2)
Polyethylenimine (PEI) is a highly effective gene delivery vector, but because it is an off-the shelf material, its properties may not be optimal. To investigate the effects of the protonation properties of the polymer, we generated PEI derivatives b