Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Ron Stewart"'
Autor:
Rhonda Bacher, Ning Leng, Li-Fang Chu, Zijian Ni, James A. Thomson, Christina Kendziorski, Ron Stewart
Publikováno v:
BMC Bioinformatics, Vol 19, Iss 1, Pp 1-10 (2018)
Abstract Background High-throughput expression profiling experiments with ordered conditions (e.g. time-course or spatial-course) are becoming more common for studying detailed differentiation processes or spatial patterns. Identifying dynamic change
Externí odkaz:
https://doaj.org/article/110d9b30efdc4b4a864f6430b8de2faa
Autor:
Srikumar Sengupta, Victor Ruotti, Jennifer Bolin, Angela Elwell, Alvaro Hernandez, James A. Thomson, Ron Stewart
Publikováno v:
BioTechniques, Vol 49, Iss 6, Pp 898-904 (2010)
Preparation of an Illumina sequencing library for gene expression analysis (mRNA-Seq) requires microgram amounts of starting total RNA or PCR-based amplification. Here we describe a protocol based on T7 linear RNA amplification that does not introduc
Externí odkaz:
https://doaj.org/article/6165fa2691f84925b59335ab3a3ddb81
Autor:
Matthew N. Bernstein, Mark E. Burkard, Zijian Ni, Michael Collins, Ron Stewart, Christina Kendziorski
Publikováno v:
BMC Bioinformatics, Vol 22, Iss 1, Pp 1-9 (2021)
BMC Bioinformatics
BMC Bioinformatics
Background Single-cell RNA-seq (scRNA-seq) enables the profiling of genome-wide gene expression at the single-cell level and in so doing facilitates insight into and information about cellular heterogeneity within a tissue. This is especially importa
Autor:
Michael P. Schwartz, Amy Van Aartsen, Christina Kendziorski, Cara Argus, Matthew T. Schmitz, Christopher Barry, Scott Swanson, Jared Brown, Ron Stewart, James A. Thomson, Jennifer M. Bolin, John Steill
Publikováno v:
PLoS Computational Biology
PLoS Computational Biology, Vol 17, Iss 3, p e1008778 (2021)
PLoS Computational Biology, Vol 17, Iss 3, p e1008778 (2021)
Human pluripotent stem cells hold significant promise for regenerative medicine. However, long differentiation protocols and immature characteristics of stem cell-derived cell types remain challenges to the development of many therapeutic application
Autor:
Michael Collins, Mark E. Burkard, Zijian Ni, Matthew N. Bernstein, Christina Kendziorski, Ron Stewart
BackgroundSingle-cell RNA-seq (scRNA-seq) enables the profiling of genome-wide gene expression at the single-cell level and in so doing facilitates insight into and information about cellular heterogeneity within a tissue. Perhaps nowhere is this mor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6283131f79c244a6ed667727834dbdeb
https://doi.org/10.1101/2020.09.23.310441
https://doi.org/10.1101/2020.09.23.310441
Autor:
Scott Swanson, Christina Kendziorski, Brian E. McIntosh, Ning Leng, Bret Duffin, James A. Thomson, Ron Stewart, Jennifer M. Bolin, Cara Argus, Christopher Barry, Rhonda Bacher, Mitchell D. Probasco, John Steill, Matthew T. Schmitz
Publikováno v:
PLoS Computational Biology, Vol 15, Iss 12, p e1007543 (2019)
PLoS Computational Biology
PLoS Computational Biology
Pluripotent stem cells retain the developmental timing of their species of origin in vitro, an observation that suggests the existence of a cell-intrinsic developmental clock, yet the nature and machinery of the clock remain a mystery. We hypothesize
Autor:
Tania Incitti, Fabrizio Rinaldi, James A. Thomson, Radbod Darabi, Ami Yamamoto, Scott Swanson, Ron Stewart, James Kiley, Rita C.R. Perlingeiro, Alessandro Magli, Jakub Tolar, Sridhar Selvaraj, Ce Yuan
Publikováno v:
Cell Reports, Vol 19, Iss 13, Pp 2867-2877 (2017)
Summary Pluripotent stem (PS)-cell-derived cell types hold promise for treating degenerative diseases. However, PS cell differentiation is intrinsically heterogeneous; therefore, clinical translation requires the development of practical methods for
Autor:
Yongsheng Bai, Victor Ruotti, Bao Kim Nguyen, James A. Thomson, Ron Stewart, Zhonggang Hou, Angela L. Elwell, Scott Swanson, William L. Murphy, Michael P. Schwartz, Hamisha Ardalani, John Steill, Jennifer M. Bolin, Jue Zhang
Publikováno v:
Stem Cell Reports, Vol 8, Iss 4, Pp 907-918 (2017)
Stem Cell Reports
Stem Cell Reports
Summary A defined protocol for efficiently deriving endothelial cells from human pluripotent stem cells was established and vascular morphogenesis was used as a model system to understand how synthetic hydrogels influence global biological function c
Autor:
Lauren J. Mills, James A. Thomson, Scott Swanson, Bridget S. Dillon, David A. Stafford, Alessandro Magli, Rita C.R. Perlingeiro, Ricardo Mondragón González, Il Youp Kwak, Ron Stewart, June Baik, Daniel J. Garry, Brian David Dynlacht
Publikováno v:
PLoS Biology, Vol 17, Iss 2, p e3000153 (2019)
PLoS Biology
PLoS Biology
The transcriptional mechanisms driving lineage specification during development are still largely unknown, as the interplay of multiple transcription factors makes it difficult to dissect these molecular events. Using a cell-based differentiation pla
Publikováno v:
Nucleic Acids Research
Comparative time series transcriptome analysis is a powerful tool to study development, evolution, aging, disease progression and cancer prognosis. We develop TimeMeter, a statistical method and tool to assess temporal gene expression similarity, and