Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Rong Fu Wang"'
Publikováno v:
Experimental Gerontology. 33:239-247
The effect of a food-restricted diet on the fecal microflora of rats was studied by determining total anaerobic bacteria, bacterial cellular fatty acids, and the predominant intestinal bacteria shown by polymerase chain reaction (PCR) primers specifi
Publikováno v:
Applied and Environmental Microbiology. 62:1825-1830
The 2,3-dihydroxybiphenyl 1,2-dioxygenase (2,3-DBPD) of Pseudomonas putida OU83 was constitutively expressed and purified to apparent homogeneity. The apparent molecular mass of the native enzyme was 256 kDa, and the subunit molecular mass was 32 kDa
Publikováno v:
Applied and Environmental Microbiology. 62:1242-1247
PCR procedures based on 16S rRNA gene sequences specific for 12 anaerobic bacteria that predominate in the human intestinal tract were developed and used for quantitative detection of these species in human (adult and baby) feces and animal (rat, mou
Autor:
Wei-Wen Cao, Ashraf A. Khan, Daniel R. Doerge, Carl E. Cerniglia, David Wennerstrom, Rong-Fu Wang
Publikováno v:
Applied and environmental microbiology. 67(8)
Mycobacterium sp. strain PYR-1 degrades high-molecular-weight polycyclic hydrocarbons (PAHs) primarily through the introduction of both atoms of molecular oxygen by a dioxygenase. To clone the dioxygenase genes involved in PAH degradation, two-dimens
Autor:
Michael F. Bucker, Sean Kelly, Sharon L. Friedman, Donald D. Paine, Steve Madzo, Warren L. Campbell, Michael J. Myers, Denise A. Dove-Pettit, Carl E. Cerniglia, Dorothy E. Farrell, Rong-Fu Wang
Publikováno v:
Journal of food protection. 64(4)
This study validated a polymerase chain reaction-based method for the detection of a specific bovine mitochondrial gene derived from rendered bovine tissues and admixed with complete animal feed. Four laboratories participated in this effort: one sta
A 81-kDa protein from Mycobacterium sp. strain PYR-1 was expressed in response to exposure of the strain to the polycyclic aromatic hydrocarbon pyrene and recovered by two-dimensional gel electrophoresis. The N-terminal sequence of the protein indica
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::554a603f80c953b198717986b12547a6
https://europepmc.org/articles/PMC92299/
https://europepmc.org/articles/PMC92299/
Publikováno v:
Applied and environmental microbiology. 58(9)
A rapid polymerase chain reaction (PCR) method was developed for detection of Listeria monocytogenes in foods. This method used a pair of primers based on a unique region in the 16S rRNA sequence of L. monocytogenes, which were previously reported by
Publikováno v:
Applied and environmental microbiology. 57(12)
Crude rRNA was isolated from Listeria monocytogenes, L. innocua, and L. ivanovii and sequenced by a reverse transcriptase method. Only two sequence regions were found to differ for L. monocytogenes versus L. innocua or L. ivanovii. Two oligonucleotid