Zobrazeno 11 - 20
of 51
pro vyhledávání: '"Richard A, Koup"'
Autor:
Mario Roederer, Richard A. Koup, David A. Ambrozak, Melody G. Duvall, Sarah Rowland-Jones, Assan Jaye, Melissa L. Precopio, Andrew J. McMichael, Hilton Whittle
Publikováno v:
European Journal of Immunology. 38:350-363
HIV-2 is distinguished clinically and immunologically from HIV-1 infection by delayed disease progression and maintenance of HIV-specific CD4(+) T cell help in most infected subjects. Thus, HIV-2 provides a unique natural human model in which to inve
Autor:
Jian Li, Chang-You Wu, Ling-yun Wang, Richard A. Koup, Robert T. Bailer, Li-tao Yang, Hui Peng, Zhi-qiang Lu
Publikováno v:
Microbes and Infection
E protein is a membrane component of severe acute respiratory syndrome coronavirus (SARS-CoV). Disruption of E protein may reduce viral infectivity. Thus, the SARS-CoV E protein is considered a potential target for the development of antiviral drugs.
Publikováno v:
Nature Protocols. 1:1507-1516
We describe here a method for optimizing the use of polychromatic flow cytometry (with up to 17 fluorochromes simultaneously) in surface and intracellular staining of human T lymphocytes. We will highlight and discuss how to procedurally optimize key
Autor:
David R. Ambrozak, Mario Roederer, Pratip K. Chattopadhyay, Richard A. Koup, Richard Nguyen, Laurie Lamoreaux, Stephen P. Perfetto
Publikováno v:
Journal of Immunological Methods. 313:199-208
Membrane-damaged cells caused by either mechanical trauma or through normal biological processes can produce artifacts in immunophenotyping analysis by flow cytometry. Dead cells can nonspecifically bind monoclonal antibody conjugates, potentially le
Autor:
Rafael Solana, Sara Ferrando-Martinez, Juan Delgado, Beatriz Dominguez-Molina, Joseph P. Casazza, M. Ángeles Muñoz-Fernández, Manuel Leal, Rafael de la Rosa, Rebeca S. de Pablo-Bernal, Richard A. Koup, Ezequiel Ruiz-Mateos
Persistent cytomegalovirus (CMV) infection has been suggested to be a major driving force in the immune deterioration and an underlying source of age-related diseases in the elderly. CMV antibody titers are associated with lower responses to vaccinat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1e08d0ca4459287cb89515a7572f253
https://europepmc.org/articles/PMC4612356/
https://europepmc.org/articles/PMC4612356/
Publikováno v:
The Journal of Experimental Medicine
Dendritic cells (DCs) are essential antigen-presenting cells for the induction of T cell immunity against pathogens such as human immunodeficiency virus (HIV)-1. At the same time, HIV-1 replication is strongly enhanced in DC–T cell clusters, potent
Autor:
Mario Roederer, Fabien X. Lü, Richard A. Koup, Stephen P. Perfetto, Susan Moser, Joanne Yu, Judith Falloon, Christopher J. Miller, Thomas G. Evans, Stephen C. De Rosa
Publikováno v:
The Journal of Immunology. 173:5372-5380
In recent years, the quantification of T cell responses to pathogens or immunogens has become a common tool in the evaluation of disease pathogenesis or vaccine immunogenicity. Such measurements are usually limited to enumerating IFN-γ-producing cel
Autor:
John Hegarty, Daniel C. Douek, Jacinta Kelly, Cliona O'Farrelly, Richard A. Koup, Lucy Golden-Mason
Publikováno v:
The Journal of Immunology. 172:5980-5985
Normal adult human liver (AHL) contains populations of unconventional lymphocytes that have been shown in the mouse to mature locally. The presence of lymphoid progenitors together with IL-7, recombinase-activating gene, and pre-TCR-α expression in
Publikováno v:
The Journal of Immunology. 172:2219-2224
Langerhans cells (LC) are likely initial targets for HIV following sexual exposure to virus and provide an efficient means for HIV to gain access to lymph node T cells. The purpose of this study was to examine the nature of the CD4+ T cell that becom
Autor:
Richard A. Koup, Stephen C. De Rosa, Jason M. Brenchley, Daniel C. Douek, Michael R. Betts, David Price, Mario Roederer
Publikováno v:
Journal of Immunological Methods. 281:65-78
Flow cytometric detection of antigen-specific CD8+ T cells has previously been limited to MHC-class I tetramer staining or intracellular cytokine production, neither of which measure the cytolytic potential of these cells. Here we present a novel tec