Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Richard A, Koup"'
Autor:
Li Li, Yan-Ying Fan, Richard A. Koup, Tao Yu, Zi-Tong Huang, Robert T. Bailer, Man-Hui Wu, Chang-You Wu
Publikováno v:
Archives of Virology
SARS-CoV infection of human results in antigen-specific cellular and humoral immune responses. However, it is critical to determine whether SARS-CoV-specific memory T cells can persist for long periods of time. In this study, we analyzed the cellular
Autor:
Mario Roederer, Richard A. Koup, David A. Ambrozak, Melody G. Duvall, Sarah Rowland-Jones, Assan Jaye, Melissa L. Precopio, Andrew J. McMichael, Hilton Whittle
Publikováno v:
European Journal of Immunology. 38:350-363
HIV-2 is distinguished clinically and immunologically from HIV-1 infection by delayed disease progression and maintenance of HIV-specific CD4(+) T cell help in most infected subjects. Thus, HIV-2 provides a unique natural human model in which to inve
Autor:
Jian Li, Chang-You Wu, Ling-yun Wang, Richard A. Koup, Robert T. Bailer, Li-tao Yang, Hui Peng, Zhi-qiang Lu
Publikováno v:
Microbes and Infection
E protein is a membrane component of severe acute respiratory syndrome coronavirus (SARS-CoV). Disruption of E protein may reduce viral infectivity. Thus, the SARS-CoV E protein is considered a potential target for the development of antiviral drugs.
Publikováno v:
Nature Protocols. 1:1507-1516
We describe here a method for optimizing the use of polychromatic flow cytometry (with up to 17 fluorochromes simultaneously) in surface and intracellular staining of human T lymphocytes. We will highlight and discuss how to procedurally optimize key
Autor:
Michael R. Betts, Richard A. Koup, David R. Ambrozak, Jason M. Brenchley, Daniel C. Douek, L. S. Davis, M. Chatterji
Publikováno v:
Clinical and Experimental Immunology. 130:432-440
Summary Naïve T-cells divide and mature, both functionally and phenotypically, upon stimulation through the T-cell receptor. Although much is known about the overall changes that occur in naïve cells upon TCR stimulation, and the different memory/e
Autor:
Takuya Yamamoto, Ross W. B. Lindsay, Francois Lefebvre, Richard A. Koup, Rafick Pierre Sekaly, Emma Gostick, Mark J. Cameron, Elias K. Haddad, Jason G. D. Gall, David Price, Robert A. Seder, Matthew Johnson, Karin Loré, Constantinos Petrovas
Publikováno v:
Journal of immunology (Baltimore, Md. : 1950). 188(12)
Recombinant adenovirus (rAd) vectors are being investigated as vaccine delivery vehicles in preclinical and clinical studies. rAds constructed from different serotypes differ in receptor usage, tropism, and ability to activate cells, aspects of which
Autor:
Laurie Lamoreaux, Richard A. Koup, Stephen P. Perfetto, Pratip K. Chattopadhyay, David R. Ambrozak, Mario Roederer, Richard Nguyen
Publikováno v:
Current Protocols in Cytometry
Amine-reactive dyes, also known as LIVE/DEAD fixable dead cell stains, are a class of viability dyes suitable for identifying dead cells in samples that will be fixed. These dyes cross the cell membranes of dead cells, and react with free amines in t
Autor:
Richard A Koup, Mario Roederer, Laurie Lamoreaux, Jennifer Fischer, Laura Novik, Martha C Nason, Brenda D Larkin, Mary E Enama, Julie E Ledgerwood, Robert T Bailer, John R Mascola, Gary J Nabel, Barney S Graham, VRC 009 Study Team, VRC 010 Study Team
Publikováno v:
PLoS ONE, Vol 5, Iss 2, p e9015 (2010)
PLoS ONE
PLoS ONE
Background Induction of HIV-1-specific T-cell responses relevant to diverse subtypes is a major goal of HIV vaccine development. Prime-boost regimens using heterologous gene-based vaccine vectors have induced potent, polyfunctional T cell responses i
Autor:
David Price, Joanne Yu, Marcel P. Bruchez, Paul A. Goepfert, Mario Roederer, Emma Gostick, Richard A. Koup, Pratip K. Chattopadhyay, Theresa F. Harper, Michael R. Betts, Stephen C. De Rosa, Stephen P. Perfetto
Publikováno v:
Nature medicine. 12(8)
Immune responses arise from a wide variety of cells expressing unique combinations of multiple cell-surface proteins. Detailed characterization is hampered, however, by limitations in available probes and instrumentation. Here, we use the unique spec