Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Andrew F. Taylor"'
Publikováno v:
Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
Scientific Reports
Scientific Reports
Repair of broken DNA by homologous recombination requires coordinated enzymatic reactions to prepare it for interaction with intact DNA. The multiple activities of enterobacterial RecBCD helicase-nuclease are coordinated by Chi recombination hotspots
Publikováno v:
Nucleic Acids Research
Homologous recombination occurs especially frequently near special chromosomal sites called hotspots. In Escherichia coli, Chi hotspots control RecBCD enzyme, a protein machine essential for the major pathway of DNA break-repair and recombination. Re
Autor:
Jeffrey A. Ranish, Andrew F. Taylor, Jie Luo, Kelly K. Lee, Susan K. Amundsen, Gerald R. Smith, Miklos Guttman
Publikováno v:
Journal of Molecular Biology. 426:3479-3499
Faithful repair of DNA double-strand breaks by homologous recombination is crucial to maintain functional genomes. The major Escherichia coli pathway of DNA break repair requires RecBCD enzyme, a complex protein machine with multiple activities. Upon
Autor:
Andrew F. Taylor, Aaron L. Lucius, Janid A. Ali, Alessandro Vindigni, Timothy M. Lohman, Gerald R. Smith, Razmic Gregorian
Publikováno v:
Journal of Molecular Biology. 324:409-428
The mechanism by which Escherichia coli RecBCD DNA helicase unwinds duplex DNA was examined in vitro using pre-steady-state chemical quenched-flow kinetic methods. Single turnover DNA unwinding experiments were performed by addition of ATP to RecBCD
Publikováno v:
Genetics. 161:483-492
The heterotrimeric RecBCD enzyme of Escherichia coli is required for the major pathway of double-strand DNA break repair and genetic exchange. Assembled as a heterotrimer, the enzyme has potent nuclease and helicase activity. Analysis of recC nonsens
Autor:
Andrew F. Taylor, Gerald R. Smith
Publikováno v:
Journal of Biological Chemistry. 270:24451-24458
RecBCD enzyme is a multifunctional nuclease that is essential for the major pathway of homologous genetic recombination in Escherichia coli. It has a potent helicase activity that uses ATP hydrolysis to unwind very long stretches of DNA. The function
Autor:
Andrew F. Taylor, Gerald R. Smith
Publikováno v:
Nature. 423(6942)
Helicases are molecular motors that move along and unwind double-stranded nucleic acids. RecBCD enzyme is a complex helicase and nuclease, essential for the major pathway of homologous recombination and DNA repair in Escherichia coli. It has sets of
Autor:
Andrew F. Taylor
Publikováno v:
Cell. 69(7)
RuvA and RuvB act together to move Holliday junctions. RuvC cleaves Holliday junctions and apparently acts in concert with RuvA and RuvB. RecG can substitute for RuvABC in the RecBCD pathway of recombination but not in the RecF pathway.
Publikováno v:
Cell. 41:145-151
Chi sites enhance in their vicinity homologous recombination by the E. coli RecBC pathway. We report here that RecBC enzyme catalyzes Chi-dependent cleavage of one DNA strand, that containing the Chi sequence 5'G-C-T-G-G-T-G-G3'. Chi-specific cleavag
Publikováno v:
Journal of Bacteriology. 155:664-680
Pseudorevertants of an Escherichia coli exonuclease V (RecBC enzyme)-negative mutant have been isolated after ethyl methane sulfonate mutagenesis of a recC73 (presumed missense) mutant. The remedial mutations in each of the four pseudorevertants stud