Zobrazeno 1 - 4
of 4
pro vyhledávání: '"Lewis Y. Geer"'
Autor:
Joshua J. Coon, Neil G. Rumachik, Graeme C. McAlister, Michael S. Westphall, David J. Pagliarini, Jason D. Russell, Alexander S. Hebert, John E. P. Syka, Lewis Y. Geer
Publikováno v:
Analytical Chemistry. 84:2875-2882
We describe the first implementation of negative electron-transfer dissociation (NETD) on a hybrid ion trap-orbitrap mass spectrometer and its application to high-throughput sequencing of peptide anions. NETD, coupled with high pH separations, negati
Publikováno v:
International Journal of Mass Spectrometry. 259:197-203
Direct analysis of intact proteins on a chromatographic time scale is demonstrated on a modified linear ion trap mass spectrometer using sequential ion/ion reactions, electron transfer and proton transfer, to dissociate the sample and to convert the
Autor:
Viswanadham Sridhara, Stephen H. Bryant, An Chi, Dina L. Bai, Jeffrey Shabanowitz, Donald F. Hunt, Lewis Y. Geer
Publikováno v:
Proteome Science
Proteome Science, Vol 10, Iss 1, p 8 (2012)
Proteome Science, Vol 10, Iss 1, p 8 (2012)
Background Electron Transfer Dissociation [ETD] can dissociate multiply charged precursor polypeptides, providing extensive peptide backbone cleavage. ETD spectra contain charge reduced precursor peaks, usually of high intensity, and whose pattern is
Autor:
Dina L. Bai, Donald F. Hunt, Daniel J. Burke, Joshua J. Coon, Lewis Y. Geer, Olga G. Troyanskaya, John E. P. Syka, An Chi, Jeffrey Shabanowitz, Curtis Huttenhower
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 104(7)
We present a strategy for the analysis of the yeast phosphoproteome that uses endo-Lys C as the proteolytic enzyme, immobilized metal affinity chromatography for phosphopeptide enrichment, a 90-min nanoflow-HPLC/electrospray-ionization MS/MS experime