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pro vyhledávání: '"Fiona Flett"'
Autor:
Uwe Rauland, Pierre Leblond, Ralf Kliem, Oliver Wafzig, Fiona Flett, Matthias Redenbach, Wolfang Piendl, John Cullum, Ingrun Glocker
Publikováno v:
Molecular and General Genetics MGG
Molecular and General Genetics MGG, Springer Verlag, 1993, 241 (3-4), pp.255-62
Molecular and General Genetics MGG, Springer Verlag, 1993, 241 (3-4), pp.255-62
International audience; Genetic instability in Streptomyces species often involves large deletions sometimes accompanied by DNA amplification. Two such systems in Streptomyces lividans 66 involve the production of mutants sensitive to chloramphenicol
Publikováno v:
Journal of basic microbiology. 27(1)
Streptomyces coelicolor A3(2) gives rise to spontaneous chloramphenicol sensitive mutants at a frequency of about 0.3% per spore. These mutants are often genetically unstable and give rise to arginine auxotrophs (Arg-) at frequencies of 1-7% per spor
Publikováno v:
Biotechnologygenetic engineering reviews. 4
Genetic instability is very common in Streptomyces species, but only affects specific genes in any one strain. It sometimes occurs at high frequency spontaneously, but may be stimulated by treatments such as UV irradiation or intercalating agents. De
Autor:
Fiona Flett, John Cullum
Publikováno v:
Moleculargeneral genetics : MGG. 207(2-3)
A mutant of Streptomyces coelicolor A 3(2) highly resistant to chloramphenicol was selected. It had amplified some chromosomal DNA fragments to a copy number of 20-50. Some of the amplified fragments were cloned and used as hybridisation probes to in