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pro vyhledávání: '"Kenneth J. Livak"'
Autor:
Shuqiang, Li, Kenneth J, Livak
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1979
Single-cell sequencing of TCR alleles enables determination of T cell specificity. Here we describe a sensitive protocol for targeted amplification of TCR CDR3 regions from single-cell full-length cDNA libraries. By exploiting the specificity of RNas
Autor:
Kenneth J. Livak, Victoria L. Boyd, Achim Karger, Gerald Zon, John W. Burns, Kristina I. Moody
Publikováno v:
Analytical Biochemistry. 354:266-273
Fundamental to understanding the role of cytosine (C) methylation in genomic DNA (gDNA) is the need for robust analysis methods to determine the location and degree of this modification. We report a novel method for methylation detection by denaturin
Autor:
Kenneth J. Livak
Publikováno v:
Genetic Analysis: Biomolecular Engineering. 14:143-149
Large-scale screening for known polymorphisms will require techniques with few steps and the ability to automate each of these steps. In this regard, the 5' nuclease, or TaqMan, PCR assay is especially attractive. A fluorogenic probe, consisting of a
Publikováno v:
Genome Research. 8:549-556
Single-nucleotide variations are the most widely distributed genetic markers in the human genome. A subset of these variations, the substitution mutations, are responsible for most genetic disorders. As single nucleotide polymorphism (SNP) markers ar
Autor:
James W. Hainer, Kenneth J. Livak
Publikováno v:
Human Mutation. 3:379-385
Genotype determination using the solid-phase minisequencing method of Syvanen et al. (1990, 1993) has been adapted for use with fluorescein-labeled dideoxynucleotides (F-ddNTPs). PCR is performed using one biotinylated primer and one unbiotinylated p
Publikováno v:
Mutation research. 406(1)
Sequencing of a human DNA ligase I cDNA clone derived from HeLa cells revealed two unreported differences with the published sequence: a single base change and a three-base deletion. Both differences are in exon 6, and were analyzed by amplifying a s
A detection system based on the PCR has been developed for Escherichia coli strains which harbor the Shiga-like toxin genes. This quantitative detection system involves the 5'-->3' nuclease activity of Thermus aquaticus DNA polymerase, which cleaves
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee0482924cf959e26dabab92c00d240f
https://europepmc.org/articles/PMC167901/
https://europepmc.org/articles/PMC167901/
Publikováno v:
PCR methods and applications. 4(6)
The 5' nuclease PCR assay detects the accumulation of specific PCR product by hybridization and cleavage of a double-labeled fluorogenic probe during the amplification reaction. The probe is an oligonucleotide with both a reporter fluorescent dye and
Autor:
Mark R. Andersen, Danny H. Lee, Kaiqin Lao, Julie T. Nguyen, Nan Lan Xu, Zhaohui Zhou, Kenneth J. Livak, Dana Ridzon, Adam Broomer, Caifu Chen, Karl J. Guegler, Vikram R. Mahuvakar, Maura Barbisin
Publikováno v:
Nucleic Acids Research
A novel microRNA (miRNA) quantification method has been developed using stem–loop RT followed by TaqMan PCR analysis. Stem–loop RT primers are better than conventional ones in terms of RT efficiency and specificity. TaqMan miRNA assays are specif