Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Koichi Abe"'
Autor:
Wataru Yoshida, Kazunori Ikebukuro, Nasa Savory, Takahiro Fukaya, Koichi Abe, Koji Sode, Stefano Ferri, Kaori Tsukakoshi
Publikováno v:
Journal of Biotechnology. 212:99-105
Aptamers are mainly selected by in vitro selection using random nucleic acid libraries. These aptamers have often shown insufficient affinity for biomedical applications. We improved DNA aptamer binding affinity for vascular endothelial growth factor
Autor:
Wataru Yoshida, Kenichiro Hata, Koji Sode, Kazunori Ikebukuro, Koichi Abe, Christophe A. Marquette, Kazuhiko Nakabayashi, Hironobu Wakeda, Jinhee Lee, Loïc J. Blum
Publikováno v:
Biosensors and Bioelectronics
Biosensors and Bioelectronics, Elsevier, 2016, 93, pp.118-123. ⟨10.1016/j.bios.2016.09.060⟩
Biosensors and Bioelectronics, Elsevier, 2016, 93, pp.118-123. ⟨10.1016/j.bios.2016.09.060⟩
International audience; DNA methylation level at a certain gene region is considered as a new type of biomarker for diagnosis and its miniaturized and rapid detection system is required for diagnosis. Here we have developed a simple electrochemical d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7bea2235acc0474d26702c0bd5c40070
https://hal.archives-ouvertes.fr/hal-01483845
https://hal.archives-ouvertes.fr/hal-01483845
Autor:
Shinichi Goto, Nasa Savory, Kazunori Ikebukuro, Koichi Abe, Wataru Yoshida, Yutaka Unuma, Stefano Ferri, Makoto Nakamura
Publikováno v:
Analytical Letters. 46:2954-2963
A fully automated two-dimensional electrophoresis (2DE) system was employed for DNA aptamer selection against an unidentified protein in a mouse liver tissue extract as a model target. A 2DE-based systematic evolution of ligands by exponential enrich
Autor:
Koichi Abe, Wataru Yoshida, Kenichiro Hata, Kazunori Ikebukuro, Daisuke Hiraoka, Hironobu Wakeda
Publikováno v:
Analytical Chemistry. 84:8259-8264
DNA methylation, which is an important epigenetic event for transcriptional regulation, is regarded as a biomarker for cancer. A rapid and sensitive method for measuring DNA methylation levels in target genomic regions may enable early diagnosis of c
Publikováno v:
Biotechnology Letters. 32:1293-1298
Motif analysis of PGa4 indicated that eight consecutive guanine residues were most important for pyrroloquinoline quinine glucose dehydrogenase (PQQGDH) recognition. Because the truncated mutants minimized unexpected interactions between PGa4 and the
Publikováno v:
Journal of Microbial & Biochemical Technology.
With recent advances in nanotechnology and sequencing technology, DNA diagnostic technology is becoming practical and is advancing at a fast pace. In these detection systems, mainly PCR (particularly real-time PCR) and DNA probe hybridization techniq
Autor:
Jinhee Lee, Aki Kezuka, Koichi Abe, Hiroaki Motoki, Shizunobu Igimi, Mamoru Noda, Wataru Yoshida, Yoshiyuki Murakami, Takafumi Matsuo, Nobuaki Shimura, Kazunori Ikebukuro
Publikováno v:
Analytica chimica acta. 801
An automatic polymerase chain reaction (PCR) product detection system for food safety monitoring using zinc finger (ZF) protein fused to luciferase was developed. ZF protein fused to luciferase specifically binds to target double stranded DNA sequenc
Affinity improvement of a VEGF aptamer by in silico maturation for a sensitive VEGF-detection system
Autor:
Stefano Ferri, Till T. Bachmann, Koichi Abe, Wataru Yoshida, Holger Schulze, Kazunori Ikebukuro, Yoshihiko Nonaka
Publikováno v:
Analytical chemistry. 85(2)
Systematic evolution of ligands by exponential enrichment (SELEX) is an efficient method to identify aptamers; however, it sometimes fails to identify aptamers that bind to their target with high affinity. Thus, post-SELEX optimization of aptamers is
Autor:
Michio Horiuchi, Kazunori Ikebukuro, Koichi Abe, Aki Kezuka, Takenori Kumagai, Koji Sode, Yuko Osawa, Shizunobu Igimi, Hiroaki Motoki, Chiaki Takahashi, Yoshiyuki Murakami, Takafumi Matsuo
Publikováno v:
Analytical chemistry. 84(18)
We constructed a novel bacterial genome detection system using zinc finger protein (ZF) fused with firefly luciferase (ZF-luciferase). Taking advantage of the direct recognition of double-stranded DNA (dsDNA) by ZF, we previously constructed bacteria
Publikováno v:
Faraday discussions. 149
Aptamers are nucleic acids that can bind to various molecules. Because they have some features that are lacking in antibodies, aptamers could serve as alternatives to antibodies. For the purpose of biosensing, we focused on aptamers that undergo stru