Zobrazeno 1 - 4
of 4
pro vyhledávání: '"Christian Gaudin"'
Autor:
C Reverbel-Leroy, Christian Gaudin, Alain Bernadac, Anne Belaich, Chantal Tardif, Jean-Pierre Belaich
Publikováno v:
Microbiology. 142:1013-1023
The CelF-encoding sequence was isolated from Clostridium cellulolyticum genomic DNA using the inverse PCR technique. The gene lies between cipC (the gene encoding the cellulosome scaffolding protein) and celC (coding for the endoglucanase C) in the l
Autor:
Christian Gaudin, Anne Belaich, M Loutfi, Henri-Pierre Fierobe, Eric Faure, Chantal Bagnara, Daniel Baty, Jean-Pierre Belaich
Publikováno v:
Journal of Bacteriology. 173:7956-7962
A construction was carried out to obtain a high level of expression in Escherichia coli of the gene celCCA, coding for the endoglucanase A from Clostridium cellulolyticum (EGCCA). The enzyme was purified in two forms with different molecular weights,
Publikováno v:
Journal of bacteriology. 182(7)
CelE, one of the three major proteins of the cellulosome of Clostridium cellulolyticum , was characterized. The amino acid sequence of the protein deduced from celE DNA sequence led us to the supposition that CelE is a three-domain protein. Recombina
Autor:
Chantal Bagnara-Tardif, Christian Gaudin, Françoise Guerlesquin, Henri-Pierre Fierobe, Paul Sauve, Jean-Pierre Belaich, Anne Belaich
Publikováno v:
European journal of biochemistry. 217(2)
An Escherichia coli clone was constructed to overproduce endoglucanase C (CelCCC) from Clostridium cellulolyticum. This construction made it easier to isolate the enzyme but, as observed in the case of endoglucanase A (CelCCA) from the same organism,