Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Jamel, S."'
Autor:
Jamel S. Hamada
Publikováno v:
Journal of Chromatography A. 944:241-248
This study was undertaken to evaluate the possibility that an analytical ion-pair HPLC procedure used to determine phytic acid (IP6) and its degradation products (IP3-IP5) can be transformed to a preparative purification method. A commercial phytic a
Autor:
Jamel S. Hamada
Publikováno v:
Journal of the American Oil Chemists' Society. 77:779-784
Rice brans were treated with a protease together with a disulfide bond-breaking agent (Na2SO3) to achieve a 2–4% peptide bond hydrolysis (DH). Ultrafiltration (UF) with 3 kDa molecular weight cut-off (MWCO) membrane led to substantial loss of feed
Autor:
Jamel S. Hamada
Publikováno v:
Journal of Food Biochemistry. 23:307-321
Extraction yields of rice bran protein increased with the use of protease as the degree of hydrolysis (DH) increased, reaching 92% at a 10% DH value. Extraction of rice bran proteins was also carried out using limited proteolysis to 2% DH with Na 2 S
Publikováno v:
Journal of Chromatography A. 827:319-327
Peptides from rice bran protein were investigated because of their potential usefulness in industrial food uses. Peptides were generated from defatted rice bran by treatment with a commercial protease to 7.6% peptide bond hydrolysis. Protein hydrolys
Autor:
Jamel S. Hamada
Publikováno v:
Journal of the American Oil Chemists' Society. 73:1143-1151
Sequential precipitation with polyethylene glycol (PEG) and KCl followed by preparative anion exchange high-performance liquid chromatography was used to isolated acid phosphatases (APases) from germinating soybean seeds. KCl inhibited the PEG precip
Autor:
Jamel S. Hamada
Publikováno v:
Journal of Chromatography A. 734:195-203
Rice bran proteins were isolated using a strongly dissociating buffer containing 0.1 M acetic acid, 3 M urea, and 0.01 M cetyltrimethylammonium bromide (AUC). Proteins were eluted by AUC from a diol-caoted silica gel (30 cm×20 mm I.D.) with exclusio
Autor:
Jamel S. Hamada
Publikováno v:
Journal of Chromatography A. 702:163-172
Gel permeation and anion-exchange chromatography were used to develop methodology for large-scale production of peptidoglutaminase (PGase) from Bacillus circulans cell extract. Sample load, flow-rate and elution profiles were optimized to obtain a hi
Autor:
Jamel S. Hamada
Publikováno v:
Journal of Food Science. 56:1731-1734
Ultrafiltration (UF) of B. circulans cell extract using a 100kd hollow-fiber membrane had no effect on peptidoglutaminase (PGase) activity and allowed 34 and 26% of PGases I and II to permeate. UF, with 30kd spiral membrane, slightly increased PGase
Autor:
Jamel S. Hamada
Publikováno v:
Journal of Food Science. 56:1725-1728
A soy protein hydrolysate was deamidated with peptidoglutaminase (PGase) retained within a 30kd spiral membrane. Reactor was operated at 30°C and 6.5 L/m2/hr flux in recycle mode to 60% conversion, then in diafiltration mode for 2 hr. Time course, p
Autor:
Jamel S. Hamada
Publikováno v:
Journal of the American Oil Chemists' Society. 68:459-462
The limited deamidating ability of peptidoglutaminase (PGase) toward intact food proteins (0- 6% deamidation) can be significantly enhanced by prior protein hydrolysis and altering protein conformation by such means as moist heat. PGase deamidation i