Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Yong J. Lee"'
Thermotolerance expression in mitotic CHO cells without increased translation of heat shock proteins
Autor:
Michael J. Borrelli, Diane M. Stafford, Peter M. Corry, Yong J. Lee, Lisa A. Karczewski, Cynthia M. Rausch
Publikováno v:
Journal of Cellular Physiology. 169:420-428
The objective of this study was to unequivocally demonstrate thermotolerance expression in mammalian cells in the absence of stress-induced synthesis of heat shock proteins (HSPs). Mitotic cells were selected as an experimental system since their gen
Publikováno v:
Molecular and Cellular Biochemistry. 144:27-34
It was reported that chemical stresses such as arsenite, cadmium or salicylate fail to induce synthesis of the inducible form of HSP70 (HSP70i). We report here that exposure of cells to higher doses of these chemical treatments induced significant sy
Publikováno v:
Journal of Thermal Biology. 19:373-383
1. 1. CHO and L929 cells were used as a model system for investigating the activities of transcriptional factors involved in hsp70 synthesis. We observed that the heat shock response for hsp70 synthesis is different in CHO and L929 cells. Hsp70 and i
Publikováno v:
Journal of Thermal Biology. 18:229-236
1. 1.An electroporation system employing an oscillating electric pulse and centrifugal force was used to introduce HSP70 or anti-HSP70 antibody into Chinese hamster ovary cells. 2. 2.There was a 1.5- or 1.9-fold increase in the amount of intracellula
Autor:
Dooha Kim, Yong J. Lee
Publikováno v:
Journal of Thermal Biology. 18:41-48
1. 1.|In an effort to elucidate the mechanism by which glycerol protects cells against heat killing, the effects of glycerol on heat-induced protein aggregation were studied by employing a cell free system containing cytosolic proteins from CHO cells
Publikováno v:
Experimental Cell Research. 197:207-212
A novel electroporation system employing an oscillating electric pulse and centrifugal force was used to introduce extraneous proteins into CHO cells. Following the electrical pulse, the compression and subsequent rebound induced by the centrifugal a
Publikováno v:
Journal of Cellular Physiology. 149:202-207
We investigated whether or not a 50 kDa glycoprotein might play an important role in protein synthesis-independent thermotolerance development in CHO cells. When cells were heated for 10 min at 45.5 degrees C, they became thermotolerant to a heat tre
Publikováno v:
Biochemical and Biophysical Research Communications. 177:575-581
Protein denaturation resulting from temperatures between 42.0 degrees C and 50 degrees C has been observed and implicated as the lethal lesion for hyperthermic cell killing. A logical corollary is that protection against hyperthermic killing requires
Publikováno v:
Biochemical and Biophysical Research Communications. 172:119-125
We observed that members of two HSP families (70 and 28 kDa) preferentially redistributed into the nucleus after heating at 45.5 degrees C for 10 min. The rates of synthesis and redistribution of these proteins were different for each member of HSP f
Publikováno v:
Journal of Cellular Physiology. 144:401-407
The possible mechanism for heat protection by the protein synthesis inhibitor histidinol was investigated in CHO cells. Histidinol (HST, 5 mM), an analogue of the essential amino acid L-histidine, added for 2 hr before and during heating at 43 degree