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pro vyhledávání: '"Ken Sugo"'
Autor:
Ken Sugo, Tsuneo Okuyama
Publikováno v:
Separation Science and Technology. 53:1572-1579
We studied the one-step purification of endotoxin by hydroxyapatite chromatography using total lipids extracted from Escherichia coli by the Bligh and Dyer method. High endotoxic activity was detec...
Autor:
Ken Sugo, Tsuneo Okuyama
Publikováno v:
Separation Science and Technology. 53:389-396
Monophosphates such as the phospholipid lecithin poorly adsorb to hydroxyapatite because of anionic repulsion with hydroxyapatite phosphate groups. Here, we attempted to optimize the adsorption and...
Autor:
Ken Sugo, Mitsuhiro Ebara
Publikováno v:
PeerJ Analytical Chemistry. 2:e3
In this work, we demonstrate a simple spectrophotometry approach to more accurately quantify and measure paclitaxel (PTX) concentrations. PTX cannot be precisely quantified when mixed with an aqueous solvent, and carries the risk of undergoing crysta
Publikováno v:
Separation and Purification Technology. 103:161-166
The preparation method for the surface zinc-modified hydroxyapatite particles that we developed as immobilized metal ion affinity chromatography medium, required decantation process to remove the excess zinc ion. Therefore, large-scale production was
Publikováno v:
Separation Science and Technology. 46:1306-1312
Previously, we developed polyethylenimine-coated hydroxyapatite as a chromatography medium. This report describes a simple, safe, productive, and improved preparation method and reports further chromatographic properties. The improved polyethylenimin
Autor:
Ken Sugo, Shintaro Kobayashi, Katsumi Kawamura, Masahiro Tomita, Yae Kurosawa, Tsuneo Okuyama, Tomohiko Yoshitake
Publikováno v:
Separation and Purification Technology. 76:432-435
Histidine-tagged proteins have been purified with immobilized nickel affinity chromatography. This method, however, bears some serious disadvantages, including carcinogenicity and potential leakage of the nickel and the handling and disposal costs. W
Autor:
Shingo Rikimra, Katsumi Kawamura, Yukiko Murakami, Tsuneo Okuyama, Tetsuro Ogawa, Ken Sugo, Hirano Masahiro
Publikováno v:
Journal of Liquid Chromatography & Related Technologies. 32:407-417
Hydroxyapatite (HAp-CHT TypeII 40 µm) was coated with a stable layer of polyethylenimine (PEI). The resulting material was useful in column chromatography of acidic materials. A mixture of AMP, ADP, ATP, and adenosine could be resolved readily. The
Publikováno v:
Talanta. 85(3)
Polyethylenimine (PEI) has been widely used as a coating material to produce stationary phase for ion-exchange chromatography of biomolecules. However, a precise study of the PEI coating fraction has been lacking, despite such quantification being ve