Zobrazeno 1 - 10
of 16
pro vyhledávání: '"Alfredo Castello"'
Autor:
Caroline E. Lenz, Matthias W. Hentze, Joel I. Perez-Perri, Marko Noerenberg, Shabaz Mohammed, Alfredo Castello, Wael Kamel
Publikováno v:
Nat Protoc
Interactions between RNA-binding proteins (RBPs) and RNAs are critical to cell biology. However, methods to comprehensively and quantitatively assess these interactions within cells were lacking. RNA interactome capture (RIC) uses in vivo UV crosslin
Autor:
Alfredo Castello, Matthias W. Hentze, Jeroen Krijgsveld, Bernd Fischer, Mandy Rettel, Birgit Rogell
Publikováno v:
RNA. 23:1290-1302
RNA-binding proteins (RBPs) play essential roles in RNA biology, responding to cellular and environmental stimuli to regulate gene expression. Important advances have helped to determine the (near) complete repertoires of cellular RBPs. However, iden
Autor:
Svenja Hester, Krzysztof Kus, Cornelia Kilchert, Emily Priest, Ebru Aydin, Lidia Vasiljeva, Alfredo Castello, Oliver Rossbach, Tea Kecman, Shabaz Mohammed
Large RNA-binding complexes play a central role in gene expression and orchestrate production, function, and turnover of mRNAs. The accuracy and dynamics of RNA–protein interactions within these molecular machines are essential for their function a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8ef71188ccb20b10ec41d6dd22f34bb
https://doi.org/10.1101/748194
https://doi.org/10.1101/748194
RNA-binding proteins (RBPs) are typically thought of as proteins that bind RNA through one or multiple globular RNA-binding domains (RBDs) and change the fate or function of the bound RNAs. Several hundred such RBPs have been discovered and investiga
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b673698addb260e76982715b9b2c4fc
https://eprints.gla.ac.uk/219002/1/219002.pdf
https://eprints.gla.ac.uk/219002/1/219002.pdf
RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination
Autor:
Alfredo Castello, Christos Spanos, Nila Roy Choudhury, Peter Kubik, Juri Rappsilber, Shaun Webb, Sander Granneman, Gracjan Michlewski, Maryia Trubitsyna, Gregory Heikel, Jakub Stanislaw Nowak
Publikováno v:
BMC Biology, Vol 15, Iss 1, Pp 1-20 (2017)
BMC Biology
Choudhury, N R, Heikel, G, Trubitsyna, M, Kubik, P, Nowak, J S, Webb, S, Granneman, S, Spanos, C, Rappsilber, J, Castello, A & Michlewski, G 2017, ' RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination ', BMC Biology, vol. 15, 105, pp. 105-126 . https://doi.org/10.1186/s12915-017-0444-9
BMC Biology
Choudhury, N R, Heikel, G, Trubitsyna, M, Kubik, P, Nowak, J S, Webb, S, Granneman, S, Spanos, C, Rappsilber, J, Castello, A & Michlewski, G 2017, ' RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination ', BMC Biology, vol. 15, 105, pp. 105-126 . https://doi.org/10.1186/s12915-017-0444-9
Background TRIM25 is a novel RNA-binding protein and a member of the Tripartite Motif (TRIM) family of E3 ubiquitin ligases, which plays a pivotal role in the innate immune response. However, there is scarce knowledge about its RNA-related roles in c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b45757dcd1f7a88d814b282bdb68fc2
https://doi.org/10.1101/200410
https://doi.org/10.1101/200410
Autor:
Christian K. Frese, Sophia Foehr, Bernd Fischer, Jeroen Krijgsveld, Rastislav Horos, Matthias W. Hentze, Alfredo Castello, Anne-Marie Alleaume, Tomaz Curk
Publikováno v:
Europe PubMed Central
Molecular Cell
Molecular Cell
Summary Mammalian cells harbor more than a thousand RNA-binding proteins (RBPs), with half of these employing unknown modes of RNA binding. We developed RBDmap to determine the RNA-binding sites of native RBPs on a proteome-wide scale. We identified
Publikováno v:
Journal of Molecular Biology. 394:506-521
Cytolytic viruses abrogate host protein synthesis to maximize the translation of their own mRNAs. In this study, we analyzed the eukaryotic initiation factor (eIF) 4G requirement for translation of vesicular stomatitis virus (VSV) and vaccinia virus
Autor:
Alfredo Castello, Vanesa Madan, Iván Ventoso, Susana Molina, Miguel Angel Sanz, Luis Carrasco
Publikováno v:
Digital.CSIC. Repositorio Institucional del CSIC
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Brefeldin A is a macrolide compound that interferes with the secretory pathway and also affects protein synthesis in mammalian cells. As a result, this antibiotic impedes the maturation of viral glycoproteins of enveloped viruses and viral genome rep
Publikováno v:
Journal of Biological Chemistry. 281:33206-33216
Two isoforms of the translation initiation factor eIF4G, eIF4GI and eIF4GII, have been described in eukaryotic cells. The exact function of each isoform during the initiation of protein synthesis is still under investigation. We have developed an eff
Autor:
Jeroen Krijgsveld, Lars M. Steinmetz, Matthias W. Hentze, Alfredo Castello, Bernd Fischer, Claudia Strein, Rastislav Horos, Katrin Eichelbaum
Publikováno v:
Nature Protocols
Owing to their preeminent biological functions, the repertoire of expressed RNA-binding proteins (RBPs) and their activity states are highly informative about cellular systems. We have developed a novel and unbiased technique, called interactome capt