Zobrazeno 1 - 10
of 71
pro vyhledávání: '"Jonathan V. Sweedler"'
Publikováno v:
Anal Chem
Single cell analysis strives to probe molecular heterogeneity in morphologically similar cell populations through quantitative or qualitative measurements of genetic, proteomic, or metabolic products. Here, we applied mass analysis of single neurons
Publikováno v:
Anal Chim Acta
Single-cell metabolomics provides information on the biochemical state of an individual cell and its relationship with the surrounding environment. Characterization of metabolic cellular heterogeneity is challenging, in part due to the small amounts
Publikováno v:
Analytical Chemistry. 89:12375-12382
D-Amino acids (D-AAs) are endogenous molecules found throughout the metazoan, the functions of which remain poorly understood. Measurements of low abundance and heterogeneously distributed D-AAs in complex biological samples, such as cells and multic
Autor:
Marina C. Philip, Monika A. Makurath, Troy J. Comi, Stanislav S. Rubakhin, Jonathan V. Sweedler
Publikováno v:
Analytical Chemistry
The ability to characterize chemical heterogeneity in biological structures is essential to understanding cellular-level function in both healthy and diseased states, but these variations remain difficult to assess using a single analytical technique
Publikováno v:
Radiation Protection Dosimetry. 166:188-191
Within the first few microseconds following a charged particle traversal of a cell, numerous oxygen and nitrogen radicals are formed along the track. Presented here is a method, using capillary electrophoresis, for simultaneous measurement, within an
Autor:
Jordan T. Aerts, Charles L. Cox, Gubbi Govindaiah, Shane R. Crandall, Kathleen R. Louis, Jonathan V. Sweedler
Publikováno v:
Analytical Chemistry
The visual selection of specific cells within an ex vivo brain slice, combined with whole-cell patch clamp recording and capillary electrophoresis (CE)-mass spectrometry (MS)-based metabolomics, yields high chemical information on the selected cells.
Publikováno v:
Analytical Chemistry. 83:6810-6817
Single-cell mass spectrometry (MS) is a rapidly emerging field in metabolic investigations. The inherent chemical complexity of most biological samples poses analytical challenges when using MS platforms to measure sample content without prior chemic
Autor:
Emily G. Tillmaand, Monika A. Makurath, Jonathan V. Sweedler, Ta-Hsuan Ong, Stanislav S. Rubakhin
Technologies to assay single cells and their extracellular microenvironments are valuable in elucidating biological function, but there are challenges. Sample volumes are low, the physicochemical parameters of the analytes vary widely, and the cellul
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa101cf896155f1eeaccdbb747655730
https://europepmc.org/articles/PMC4426231/
https://europepmc.org/articles/PMC4426231/
Publikováno v:
ResearcherID
A laser-induced native fluorescence detection system optimized for analysis of indolamines and catecholamines by capillary electrophoresis is described. A hollow-cathode metal vapor laser emitting at 224 nm is used for fluorescence excitation, and th
Publikováno v:
Journal of Chromatography A. 1106:56-60
Capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection is a powerful tool for analysis of samples ranging from tissue extracts to single cells. However, accurate peak identification in electropherograms is challenging when com