Zobrazeno 1 - 10
of 47
pro vyhledávání: '"Yoshiyuki Takahashi"'
Autor:
Sung-Sik Kang, Keisuke Koyama, Weiping Huang, Masashi Nagano, Yoshiyuki Takahashi, Yinghua Yang, Yojiro Yanagawa
Publikováno v:
The Journal of Reproduction and Development
The present study aimed to establish an efficient system for bovine embryo production by in vitro fertilization (IVF) that can achieve stable normal fertilization and blastocyst developmental rates in any bull without optimization of the sperm concen
Autor:
Masashi Nagano, Sung-Sik Kang, Yoshiyuki Takahashi, Keisuke Koyama, Yojiro Yanagawa, Weiping Huang
Publikováno v:
The Journal of Veterinary Medical Science
The objective of this research was to estimate the optimal timing for fertilization to achieve proper embryonic development of in vitro-matured bovine oocytes. First, cumulus-oocyte complexes were subjected to in vitro maturation (IVM) for 14–22 hr
Autor:
Keisuke Koyama, Shogo Higaki, Seiji Katagiri, Tatsuyuki Takada, Masao Kishi, Masashi Nagano, Yoshiyuki Takahashi
Publikováno v:
Zygote (Cambridge, England). 25(1)
SummaryThe preselection of highly developmentally competent oocytes forin vitromaturation (IVM) is crucial for improving assisted reproductive technology. Although several intrinsic markers of oocyte quality are known to be closely related to the ons
Publikováno v:
The Journal of Reproduction and Development
The objective of this study was to clarify the effects of prematurational culture (pre-IVM) supplemented with 3-isobutyl-1-methylxanthine (IBMX) on nuclear and cytoplasmic maturation of in vitro-grown bovine oocytes. In experiment 1, oocytes (95 μm
Publikováno v:
Theriogenology. 80:793-799
Bovine ovaries offer a large pool of oocytes that could be used for in vitro production of embryos of genetically valuable animals. The effects of in vitro growth (IVG) culture duration (10, 12, and 14 days) on the viability and growth of bovine oocy
Publikováno v:
Theriogenology. 61:15-23
We determined the efficacy of a microdrop vitrification procedure for cryopreservation of bovine oocytes, using vitrified oocytes as cytoplasts for intraspecies and intergeneric somatic cell nucleus transfer (NT). In vitro matured bovine MII oocytes
Publikováno v:
Reproduction in Domestic Animals. 38:204-208
Mouse pronuclear stage embryos with intact slit zona pellucida (manipulated) were cultured in vitro until the hatched blastocyst stage in simplex optimized medium with higher K+ concentration (KSOM) prepared with three different water types: tap, dei
Publikováno v:
Journal of Veterinary Medical Science. 65:989-994
The effect of fusion/activation protocol on in vitro development of porcine nuclear transfer (NT) embryos constructed with foreign gene-transfected somatic cells were investigated. NT embryos were produced by using enucleated M II oocytes and enhance
Autor:
Koji Ikeda, Yoshiyuki Takahashi
Publikováno v:
Journal of Mammalian Ova Research. 19:46-51
We examined the effects of butyrolactone I (BL-I), a specific cdc2 kinase inhibitor, on the inhibition of meiotic resumption of porcine oocytes and subsequent developmental competence after somatic cell nuclear transfer (NT). Porcine follicular oocyt
Autor:
Yojiro Yanagawa, Sosuke Ofuji, Weiping Huang, Masashi Nagano, Kei Imai, Yoshiyuki Takahashi, Sung-Sik Kang, Keisuke Koyama
Publikováno v:
Zygote (Cambridge, England). 23(3)
SummaryThe aim of the present study was to clarify the efficacy of the well of the well (WOW) culture system for a small number of embryos and the effect of number of adjacent embryos in a WOW dish on blastocyst development. In conventional droplet c