Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Olga Garaschuk"'
Autor:
Olga Garaschuk, Bianca Brawek
Publikováno v:
Microglia ISBN: 9781493996575
Similar to many nonexcitable cells, microglia utilize intracellular Ca2+ signaling for the communication with each other as well as neurons and astrocytes and for triggering a magnitude of their executive functions. However, in vivo measurements of t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::03a45ed5b9b73406d45bcb3805bd694c
https://doi.org/10.1007/978-1-4939-9658-2_17
https://doi.org/10.1007/978-1-4939-9658-2_17
Publikováno v:
Europe PubMed Central
One of the challenges for modern neuroscience is to understand the rules of concerted neuronal function in vivo. This question can be addressed using noninvasive high-resolution imaging techniques like two-photon microscopy. This protocol describes a
Publikováno v:
Pfl�gers Archiv European Journal of Physiology. 445:357-365
Two-photon laser scanning microscopy has been used successfully for imaging activity-dependent changes of intracellular calcium and sodium levels. Here we introduce a simple technique for two-photon chloride imaging in intact neurons. It involves the
Publikováno v:
The Journal of Physiology. 502:13-30
1. The properties of ryanodine-sensitive Ca2+ stores in CA1 pyramidal cells were investigated in rat hippocampal slices by using whole-cell patch-clamp recordings combined with fura-2-based fluorometric digital imaging of cytoplasmic Ca2+ concentrati
Autor:
Hod Dana, Gregor Witte, Lawrence C. Rome, Olga Garaschuk, Thomas Thestrup, Douglas S. Kim, Luigi Russo, Yuri Kovalchuk, Taylor Allen, Yajie Liang, Julia Litzlbauer, Marsilius Mues, Tsai Wen Chen, Oliver Griesbeck, Yvonne Laukat, Stefan Becker, Anselm Geiger, Christian Griesinger, Georgios Kalamakis, Ingo Bartholomäus
Publikováno v:
Nature Methods
The quality of genetically encoded calcium indicators (GECIs) has improved dramatically in recent years, but high-performing ratiometric indicators are still rare. Here we describe a series of fluorescence resonance energy transfer (FRET)-based calci
Autor:
Masanobu Kano, Olga Garaschuk, Filippo Tempia, Arthur Konnerth, Claudia Schirra, Timothy Plant, Ralf Schneggenburger
Publikováno v:
Scopus-Elsevier
The Ca(2+)-permeation properties of AMPA-receptor (AMPA-R) channels in Purkinje neurons in rat cerebellar slices were studied using a combination of whole-cell patch-clamp recordings, Fura-2 fluorometry, and single-cell reverse-transcription (RT)-PCR
Autor:
Yury Kovalchuk, Olga Garaschuk
Publikováno v:
Cold Spring Harbor protocols. 2012(7)
This protocol describes a technique for high-resolution chloride imaging of living cells using a quinoline-based chloride (Cl−) indicator dye, MQAE (N-[6-methoxyquinolyl] acetoethyl ester). Bath-applied to acute brain slices, MQAE provides high-qua
Autor:
Lawrence B. Cohen, Arthur Konnerth, Olga Garaschuk, Bradley J. Baker, Marco Canepari, Ryota Homma, Dejan Zecevic, Maja Djurisic, Lei Jin, Chun X. Bleau
Publikováno v:
Methods in Molecular Biology (Clifton then Totova)
Dynamic Brain Imaging: Multi-Modal Methods and In Vivo Applications
Fahmeed Hyder. Dynamic Brain Imaging: Multi-Modal Methods and In Vivo Applications, 489, Humana Press Springer, pp.43-79, 2009, Methods in Molecular Biology, 〈10.1007/978-1-59745-543-5_3〉
Methods in Molecular Biology ISBN: 9781934115749
Methods in Molecular Biology
Fahmeed Hyder. Dynamic Brain Imaging: Multi-Modal Methods and In Vivo Applications, 489, Humana Press Springer, pp.43-79, 2009, Methods in Molecular Biology, ⟨10.1007/978-1-59745-543-5_3⟩
Dynamic Brain Imaging: Multi-Modal Methods and In Vivo Applications
Fahmeed Hyder. Dynamic Brain Imaging: Multi-Modal Methods and In Vivo Applications, 489, Humana Press Springer, pp.43-79, 2009, Methods in Molecular Biology, 〈10.1007/978-1-59745-543-5_3〉
Methods in Molecular Biology ISBN: 9781934115749
Methods in Molecular Biology
Fahmeed Hyder. Dynamic Brain Imaging: Multi-Modal Methods and In Vivo Applications, 489, Humana Press Springer, pp.43-79, 2009, Methods in Molecular Biology, ⟨10.1007/978-1-59745-543-5_3⟩
International audience; This chapter presents three examples of imaging brain activity with voltage- or calcium-sensitive dyes. Because experimental measurements are limited by low sensitivity, the chapter then discusses the methodological aspects th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa34cc7a50dd5fd0bfb062024cb088c5
http://www.hal.inserm.fr/inserm-00515416
http://www.hal.inserm.fr/inserm-00515416
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 100(12)
Two-photon calcium imaging is a powerful means for monitoring the activity of distinct neurons in brain tissue in vivo . In the mammalian brain, such imaging studies have been restricted largely to calcium recordings from neurons that were individual
1. The functional and molecular properties of NMDA receptors (NMDA-Rs) were studied in single, visually identified GABAergic medial septal neurones of the rat forebrain using patch clamp, fluorometric Ca2+ measurements and the single-cell reverse tra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b204cf0d26bd2c6155754392be04a12
https://europepmc.org/articles/PMC1159336/
https://europepmc.org/articles/PMC1159336/