Zobrazeno 1 - 10
of 29
pro vyhledávání: '"Michael J. MacDonald"'
Autor:
Luis A. Fernandez, James M. Ntambi, Israrul H. Ansari, Lucas M. O’Neill, Melissa J. Longacre, Michael J. MacDonald, Scott W. Stoker, Laura J. Zitur, Mindy A. Kendrick
Publikováno v:
Archives of Biochemistry and Biophysics. 618:32-43
Long-chain acyl-CoA synthetases (ACSLs) convert fatty acids to fatty acyl-CoAs to regulate various physiologic processes. We characterized the ACSL isoforms in a cell line of homogeneous rat beta cells (INS-1 832/13 cells) and human pancreatic islets
Autor:
Melissa J. Longacre, Siriluck Wattanavanitchakorn, Israr H. Ansari, Michael J. MacDonald, Scott W. Stoker, Mahmoud El Azzouny, Sarawut Jitrapakdee
Publikováno v:
Archives of biochemistry and biophysics. 676
Pyruvate carboxylase (PC) is an anaplerotic enzyme that supplies oxaloacetate to mitochondria enabling the maintenance of other metabolic intermediates consumed by cataplerosis. Using liquid chromatography mass spectrometry (LC-MS) to measure metabol
Sphingosine kinase 1 knockdown reduces insulin synthesis and secretion in a rat insulinoma cell line
Autor:
Scott W. Stoker, Suzanne G. Laychock, Mindy A. Kendrick, Noah R. Druckenbrod, Lucy D. Mastrandrea, Melissa J. Longacre, Michael J. MacDonald, Noaman Hasan
Publikováno v:
Archives of Biochemistry and Biophysics. 518:23-30
To evaluate the role of sphingosine kinase 1 (SphK1) in insulin secretion, we used stable transfection to knock down the expression of the Sphk1 gene in the rat insulinoma INS-1 832/13 cell line. Cell lines with lowered Sphk1 mRNA expression and SphK
Publikováno v:
Archives of Biochemistry and Biophysics. 488:100-104
Despite interest in malic enzyme(ME)s in insulin cells, mitochondrial malic enzyme (ME2) has only been studied with estimates of mRNA or with mRNA knockdown. Because an mRNA’s level does not necessarily reflect the level of its cognate enzyme, we d
Publikováno v:
Biochimica et Biophysica Acta (BBA) - General Subjects. 1780:966-972
We hypothesized that contrasting leucine with its non-metabolizable analog 2-aminobicyclo[2,2,1]heptane-2-carboxylic acid (BCH) might provide new information about metabolic pathways involved in insulin secretion. Both compounds stimulate insulin sec
Autor:
Michael J. MacDonald
Publikováno v:
Biochimica et Biophysica Acta (BBA) - General Subjects. 1619:77-88
Combinations of insulin secretagogue-derived metabolites were added to microgram amounts of mitochondria obtained from rat and mouse pancreatic islets and the INS-1 cell line, and the export of citric acid cycle intermediates was surveyed to study an
Autor:
Melissa J. Longacre, Scott W. Stoker, Laura J. Brown, Michael J. MacDonald, Mindy A. Kendrick, Noaman Hasan
Publikováno v:
Biochimica et biophysica acta. 1830(11)
There are three isocitrate dehydrogenases (IDHs) in the pancreatic insulin cell; IDH1 (cytosolic) and IDH2 (mitochondrial) use NADP(H). IDH3 is mitochondrial, uses NAD(H) and was believed to be the IDH that supports the citric acid cycle.With shRNAs
Autor:
Laura J. Brown, Michael J. MacDonald
Publikováno v:
Archives of Biochemistry and Biophysics. 326:79-84
cDNAs which encode the rat testis and pancreatic islet mitochondrial glycerol phosphate dehydrogenase (mGPD) (EC 1.1.99.5), the key enzyme of the glycerol phosphate shuttle, were recently cloned and sequenced and found to contain calmodulin-like calc
Autor:
Jesús Avila, Gabriel Santpere, Félix Hernández, Francisco J. Moreno, Michael J. MacDonald, Elena Gómez de Barreda, Ismael Santa-Maria, Isidro Ferrer
Publikováno v:
Digital.CSIC. Repositorio Institucional del CSIC
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Tau aggregation is a common feature of tauopathies such as Alzheimer disease (AD). In AD, tau assembles into fibrillar polymers; it may also be present in other aberrant aggregates, including Hirano bodies. The mechanisms leading to tau polymerizatio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f9a3fb0f8e421d0e032c9f9875a2af92
http://hdl.handle.net/10261/252436
http://hdl.handle.net/10261/252436
Publikováno v:
Archives of Biochemistry and Biophysics. 279:104-108
Glyceraldehyde phosphate, a glycolytic intermediate, and succinic acid (as its methyl ester to make it permeable to the cell), a citric acid cycle intermediate, were the only glucose metabolites of many recently tested that stimulated insulin release