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Publikováno v:
Methods
Circular RNAs with covalently linked ends are generated from many eukaryotic protein-coding genes when the pre-mRNA splicing machinery backsplices. These mature transcripts are resistant to digestion by exonucleases and typically have much longer hal
Autor:
Alejandro Gomez-Auli, Nicola Iovino, Michael Rauer, Dafne Ibarra-Morales, Mariana Schulte-Sasse, Francesco Cardamone, Leily Rabbani, Piergiuseppe Quarato, Fides Zenk, Germano Cecere
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
Nature Communications
Nature Communications, Nature Publishing Group, 2021, 12 (1), pp.7002. ⟨10.1038/s41467-021-27125-7⟩
Nature Communications, 12 (1)
Nature Communications, 2021, 12 (1), pp.7002. ⟨10.1038/s41467-021-27125-7⟩
Nature Communications
Nature Communications, Nature Publishing Group, 2021, 12 (1), pp.7002. ⟨10.1038/s41467-021-27125-7⟩
Nature Communications, 12 (1)
Nature Communications, 2021, 12 (1), pp.7002. ⟨10.1038/s41467-021-27125-7⟩
During embryogenesis, the genome shifts from transcriptionally quiescent to extensively active in a process known as Zygotic Genome Activation (ZGA). In Drosophila, the pioneer factor Zelda is known to be essential for the progression of development;
Autor:
Bernice Leung, Thomas Dodd, Chunli Yan, Jun Xu, Ivaylo Ivanov, Dong Wang, Juntaek Oh, Jina Yu
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Nature Communications
Nature Communications
Transcription-coupled repair is essential for the removal of DNA lesions from the transcribed genome. The pathway is initiated by CSB protein binding to stalled RNA polymerase II. Mutations impairing CSB function cause severe genetic disease. Yet, th
Publikováno v:
Nat Protoc
Precise control of gene expression requires the coordinated action of multiple factors at cis-regulatory elements. We recently developed single-molecule footprinting to simultaneously resolve the occupancy of multiple proteins including transcription
Autor:
Hongyan Wang, Robert G. Roeder, Zhong Chen, Yue Zhao, William Hankey, Alexandre Rosa Campos, Jiaoti Huang, Qianben Wang, Jeff Groth, Furong Huang
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Nature Communications
Nature Communications
RNA Polymerase II (Pol II) transcriptional recycling is a mechanism for which the required factors and contributions to overall gene expression levels are poorly understood. We describe an in vitro methodology facilitating unbiased identification of
Autor:
Jia-Lin Zhang, Yun-Ni Lei, Xiao-Qi Liu, Xiang Li, Ling-Ling Chen, Jia Wei, Jun Zhang, Yang Zhang, Xiang Gao, Li Yang, Fan Nan, Wei Xue
Publikováno v:
Science China Life Sciences. 64:1795-1809
Circular intronic RNAs (ciRNAs) escaping from DBR1 debranching of intron lariats are co-transcriptionally produced from pre-mRNA splicing, but their turnover and mechanism of action have remained elusive. We report that RNase H1 degrades a subgroup o
Publikováno v:
Nature Protocols. 16:4355-4381
Elongation, splicing and polyadenylation are fundamental steps of transcription, and studying their coordination requires simultaneous monitoring of these dynamic processes on one transcript. We recently developed a full-length nascent RNA sequencing
Autor:
Jonathan Zonszain, Ronna Shayevitch, Maram Hussein, Ifat Keydar, Galit Lev Maor, Gil Ast, Eran Meshorer, Dror Hollander, Matan Sorek, Luna Tammer, Ofir Hameiri, Yodfat Leader
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
Nature Communications
Nature Communications
In the earliest step of spliceosome assembly, the two splice sites flanking an intron are brought into proximity by U1 snRNP and U2AF along with other proteins. The mechanism that facilitates this intron looping is poorly understood. Using a CRISPR i
Publikováno v:
Exp Biol Med (Maywood)
Mediator is an evolutionarily conserved multi-protein complex that mediates the interaction between different proteins as a basic linker in the transcription mechanism of eukaryotes. It interacts with RNA polymerase II and participates in the process
Autor:
Satoru Fujimoto, Hiroshi Kimura, Takuya Sakamoto, Mayu Yoshikawa, Satoshi Uchino, Tamako Yamaoka, Shusuke Kasamatsu, Yuko Sato, Sachihiro Matsunaga, Takamasa Suzuki, Mio Shibuta, Noriyoshi Yagi
Publikováno v:
Communications Biology, Vol 4, Iss 1, Pp 1-10 (2021)
Communications Biology
Communications Biology
Spatiotemporal changes in general transcription levels play a vital role in the dynamic regulation of various critical activities. Phosphorylation levels at Ser2 in heptad repeats within the C-terminal domain of RNA polymerase II, representing the el