Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Jorge Cruz-Reyes"'
Autor:
Richard P. Metz, Bhaskara R. Madina, Blaine H. M. Mooers, Jorge Cruz-Reyes, Ralf Bundschuh, Vikas Kumar
Publikováno v:
RNA. 20:1142-1152
Mitochondrial mRNAs in kinetoplastids require extensive U-insertion/deletion editing that progresses 3′-to-5′ in small blocks, each directed by a guide RNA (gRNA), and exhibits substrate and developmental stage-specificity by unsolved mechanisms.
Autor:
James A. Wohlschlegel, Gokulan Kuppan, Sing-Hoi Sze, Yu-He Liang, Xinhua Ji, Bhaskara R. Madina, Laurie K. Read, Kurtis M. Downey, Ajay A. Vashisht, James C. Sacchettini, Jorge Cruz-Reyes
Publikováno v:
RNA. 17:1821-1830
The mitochondrial genome of kinetoplastids, including species of Trypanosoma and Leishmania, is an unprecedented DNA structure of catenated maxicircles and minicircles. Maxicircles represent the typical mitochondrial genome encoding components of the
Publikováno v:
RNA. 12:1292-1303
RBP16 is an abundant RNA binding protein from Trypanosoma brucei mitochondria that affects both RNA editing and stability. We report here experiments aimed at elucidating the mechanism of RBP16 function in RNA editing. In in vitro RNA editing assays,
Autor:
Alevtina G. Zhelonkina, Julie A. Law, Jorge Cruz-Reyes, Barbara Sollner-Webb, Catherine E. Huang, Vadim S. Alatortsev, Sean F. O'Hearn
Publikováno v:
RNA. 12:476-487
Trypanosome RNA editing is massive post-transcriptional U-insertion and U-deletion, which generates mature mRNA coding regions through cycles of endonuclease, terminal U transferase (TUTase) or 3′-U-exo, and ligase action. Both types of editing are
Publikováno v:
Molecular and Cellular Biology. 21:884-892
Trypanosome RNA editing is a massive processing of mRNA by U deletion and U insertion, directed by trans-acting guide RNAs (gRNAs). A U deletion cycle and a U insertion cycle have been reproduced in vitro using synthetic ATPase (A6) pre-mRNA and gRNA
Autor:
Barbara Sollner-Webb, Jorge Cruz-Reyes
Publikováno v:
Proceedings of the National Academy of Sciences. 93:8901-8906
We have studied the mechanism of accurate in vitro RNA editing of Trypanosoma brucei ATPase 6 mRNA, using four mRNA-guide RNA (gRNA) pairs that specify deletion of 2, 3, or 4 U residues at editing site 1 and mitochondrial extract. This extract not on
Publikováno v:
PLoS ONE
PLoS ONE, Vol 5, Iss 8, p e12235 (2010)
PLoS ONE, Vol 5, Iss 8, p e12235 (2010)
Mitochondrial mRNA editing in Trypanosoma brucei requires the specific interaction of a guide RNA with its cognate mRNA. Hundreds of gRNAs are involved in the editing process, each needing to target their specific editing domain within the target mes
RNA editing in Trypanosoma brucei is posttranscriptional uridylate removal/addition, generally at vast numbers of pre-mRNA sites, but to date, only single editing cycles have been examined in vitro. We here demonstrate achieving sequential cycles of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::40eaf976fa33a540f47bfc367845d137
https://europepmc.org/articles/PMC2268434/
https://europepmc.org/articles/PMC2268434/
Autor:
Alfredo J. Hernandez, Kenneth Stuart, Catherine Cifuentes-Rojas, Aswini K. Panigrahi, Jorge Cruz-Reyes, Anastasia Sacharidou
Publikováno v:
Journal of molecular biology. 381(1)
U-insertion/deletion RNA editing in the single mitochondrion of kinetoplastids, an ancient lineage of eukaryotes, is a unique mRNA maturation process needed for translation. Multisubunit editing complexes recognize many pre-edited mRNA sites and modi
Autor:
Jorge, Cruz-Reyes
Publikováno v:
Methods in enzymology. 424
Multisubunit RNA editing complexes recognize thousands of pre-mRNA sites in the single mitochondrion of trypanosomes. Specific determinants at each editing site must trigger the complexes to catalyze a complete cycle of either uridylate insertion or