Zobrazeno 1 - 10
of 33
pro vyhledávání: '"Koichi Abe"'
Autor:
Jinhee Lee, Atsuro Tatsumi, Kaori Tsukakoshi, Ellie D. Wilson, Koichi Abe, Koji Sode, Kazunori Ikebukuro
Publikováno v:
Sensors, Vol 20, Iss 14, p 3878 (2020)
Aptamer-based electrochemical sensors have gained attention in the context of developing a diagnostic biomarker detection method because of their rapid response, miniaturization ability, stability, and design flexibility. In such detection systems, e
Externí odkaz:
https://doaj.org/article/71706c73448743a4b1e13d05a5a66063
Publikováno v:
Materials, Vol 7, Iss 2, Pp 1046-1054 (2014)
We have developed a bound/free separation system using a vascular endothelial growth factor (VEGF) aptamer and a peptide nucleic acid (PNA) to detect VEGF. In this system, we designed capture PNA (CaPNA), which hybridizes with the aptamer in the abse
Externí odkaz:
https://doaj.org/article/ae212318bbec4b038c722ac2c3d8510c
Publikováno v:
Molecules, Vol 21, Iss 4, p 421 (2016)
Aptamers are single stranded oligonucleotides that bind a wide range of biological targets. Although aptamers can be isolated from pools of random sequence oligonucleotides using affinity-based selection, aptamers with high affinities are not always
Externí odkaz:
https://doaj.org/article/5eb58b5e816c44529b452b24a28be7e4
Autor:
Wataru Yoshida, Nasa Savory, Kaori Tsukakoshi, Koichi Abe, Kazunori Ikebukuro, Tomomi Yokoyama, Taiki Saito, Kentaro Teramoto
Publikováno v:
Biotechnology and Bioengineering. 114:2196-2203
We describe the selection of aptamers based on bioinformatics-based approaches without Systematic Evolution of Ligands by EXponential enrichment (SELEX). SELEX is a potent method; however, it is time intensive and the PCR-amplification step, which is
Autor:
Yoshio Kato, Kazunori Ikebukuro, Kaori Tsukakoshi, Chikashi Nakamura, Jinhee Lee, Koichi Abe, Ayana Yamagishi, Daisuke Matsumoto, Maui Nishio
Publikováno v:
Biosensors and Bioelectronics. 93:26-31
Genome editing with site-specific nucleases (SSNs) can modify only the target gene and may be effective for gene therapy. The main limitation of genome editing for clinical use is off-target effects; excess SSNs in the cells and their longevity can c
Autor:
Koji Sode, Koichi Abe, Kazunori Ikebukuro, Yue Ma, Wataru Yoshida, Kaori Tsukakoshi, Kazuo Nagasawa, Keisuke Iida, Yuri Ikuta
Publikováno v:
Chemical Communications. 52:12646-12649
We report that a G-quadruplex-specific compound induced structural changes in G-quadruplex-forming DNA aptamers and stabilized the folded structures, making them suitable for targeted proteins and enhancing their binding properties.
Autor:
Wataru Yoshida, Kazunori Ikebukuro, Nasa Savory, Takahiro Fukaya, Koichi Abe, Koji Sode, Stefano Ferri, Kaori Tsukakoshi
Publikováno v:
Journal of Biotechnology. 212:99-105
Aptamers are mainly selected by in vitro selection using random nucleic acid libraries. These aptamers have often shown insufficient affinity for biomedical applications. We improved DNA aptamer binding affinity for vascular endothelial growth factor
Autor:
Kazunori Ikebukuro, Nasa Savory, Stefano Ferri, Cinzia Dedi, Wataru Yoshida, Brian V. Jones, Jonathan Nzakizwanayo, Koichi Abe
Publikováno v:
Journal of Microbiological Methods. 104:94-100
In order to better control nosocomial infections, and facilitate the most prudent and effective use of antibiotics, improved strategies for the rapid detection and identification of problematic bacterial pathogens are required. DNA aptamers have much
This chapter describes Systematic evolution of ligands by exponential enrichment (SELEX) strategies with methodological improvements, well established since the first application in aptamer development. It presents a brief description of post-SELEX i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::63070a5fbf30547cef2d1812590178cc
https://doi.org/10.1201/b20047-1
https://doi.org/10.1201/b20047-1
Publikováno v:
Expert Review of Molecular Diagnostics. 14:143-151
Since aptamers were reported in 1990, research into the applications of aptamers, particularly diagnostic applications, has been growing. Aptamers can act as recognition elements instead of antibodies. In this regard, aptamers have unique characteris