Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Tadazumi Komiyama"'
Autor:
M. Enamul Kabir, Tadazumi Komiyama, Masahiko Miyamoto, Mahbubur Rahman, Yasuhiro Furuichi, Senthilkumar Krishnaswamy
Publikováno v:
Journal of Molecular Recognition. 24:631-641
Existing antifungal drugs are notable for their inability to act rapidly, as well as their toxicity and limited spectrum. The identification of fungal-specific genes and virulence factors would provide targets for new and influential drugs. The displ
Autor:
Masahiko Miyamoto, Tadazumi Komiyama, Yasuhiro Furuichi, M. Enamul Kabir, Mahbubur Rahman, Senthilkumar Krishnaswamy
Publikováno v:
Journal of Immunological Methods. 366:60-68
Aspergillus fumigatus causes the highly lethal form of invasive aspergillosis (IA). In the present study to develop a novel anti-fungal drug for protection against invasive disease, we identified a single chain fragment variable (scFv) antibody (scFv
Autor:
M. Enamul Kabir, Tadazumi Komiyama, Senthilkumar Krishnaswamy, Yasuhiro Furuichi, Masahiko Miyamoto
Publikováno v:
Journal of Biochemistry. 147:723-733
We reported previously competitive panning elution with PBS (pH 7.0) that contains HM-1 killer toxin (HM-1) and Candida albicans membrane fraction (CaMF) to release phages bound with CaMF as an antigen. Here, as an alternative strategy, we isolated h
Publikováno v:
The Journal of Antibiotics. 59:73-79
Recombinant single-chain fragment variable (scFv) anti-idiotypic antibodies were produced to represent the internal image of a HM-1 killer toxin, which is characterized by a wide spectrum of anti-fungal activity through inhibiting beta-1,3-glucan syn
Autor:
Dakshnamurthy Selvakumar, Nurul Karim, Tadazumi Komiyama, Masahiko Miyamoto, Yasuhiro Furuichi
Publikováno v:
Biological and Pharmaceutical Bulletin. 29:1848-1853
Recombinant single-chain fragment variable anti-idiotypic antibodies were produced to represent the internal image of HM-1 killer toxin and were used as novel and effective antifungal agents to inhibit in vitro beta-1,3-glucan synthase and cell growt
Autor:
M Enamul, Kabir, Senthilkumar, Krishnaswamy, Dakshnamurthy, Selvakumar, Masahiko, Miyamoto, Yasuhiro, Furuichi, Tadazumi, Komiyama
Publikováno v:
Microbiology and immunology. 58(9)
Immunoassay designs rely on the great specificity of antibodies and a suitable marker that facilitates generation of a quantitative signal. Currently, there is no reliable method for measuring the titers of an anti-idiotypic antibody. Our initial att
Autor:
Senthilkumar Krishnaswamy, Masahiko Miyamoto, Dakshnamurthy Selvakumar, M. Enamul Kabir, Nurul Karim, Yasuhiro Furuichi, Tadazumi Komiyama
Publikováno v:
Applied microbiology and biotechnology. 92(6)
Based on anti-idiotypic network theory in light of the need for new antifungal drugs, we attempted to identify biologically active fragments from HM-1 yeast killer toxin and its anti-idiotypic antibody and to compare their potency as an antifungal ag
Autor:
M. Enamul Kabir, Senthilkumar Krishnaswamy, Masahiko Miyamoto, Tadazumi Komiyama, Yasuhiro Furuichi
Publikováno v:
Applied microbiology and biotechnology. 90(2)
Phage-display and competitive panning elution leads to the identification of minimum-sized antigen binders together with conventional antibodies from a mouse cDNA library constructed from HM-1 killer toxin neutralizing monoclonal antibody (nmAb-KT).
Autor:
Masahiko Miyamoto, Senthilkumar Krishnaswamy, Yasuhiro Furuichi, Tadazumi Komiyama, M. Enamul Kabir
Publikováno v:
Analytical biochemistry. 395(1)
Phage display and two competitive panning elution conditions were used to isolate Candida-specific single chain fragment variable (scFv) antibodies. An scFv phage library constructed from splenic lymphocytes of mice immunized by idiotypic vaccination
Publikováno v:
Antimicrobial agents and chemotherapy. 50(9)
Single-chain variable-fragment (scFv) anti-idiotypic antibodies of an HM-1 killer toxin (HM-1) from the yeastWilliopsis saturnusvar.mrakiiIFO 0895 have been produced by recombinant DNA technology from the splenic lymphocytes of mice immunized by idio