Zobrazeno 1 - 10
of 82
pro vyhledávání: '"Michael J. MacDonald"'
Publikováno v:
Diabetes. 70:1575-1580
Mitochondrial glycerol phosphate dehydrogenase (mGPD) is the rate-limiting enzyme of the glycerol phosphate redox shuttle. It was recently claimed that metformin, a first-line drug used for the treatment of type 2 diabetes, inhibits liver mGPD 30-50%
Autor:
Luis A. Fernandez, James M. Ntambi, Israrul H. Ansari, Lucas M. O’Neill, Melissa J. Longacre, Michael J. MacDonald, Scott W. Stoker, Laura J. Zitur, Mindy A. Kendrick
Publikováno v:
Archives of Biochemistry and Biophysics. 618:32-43
Long-chain acyl-CoA synthetases (ACSLs) convert fatty acids to fatty acyl-CoAs to regulate various physiologic processes. We characterized the ACSL isoforms in a cell line of homogeneous rat beta cells (INS-1 832/13 cells) and human pancreatic islets
Publikováno v:
Molecular Endocrinology. 29:396-410
Pancreatic β-cells with severely knocked down cytosolic malic enzyme (ME1) and mitochondrial NAD(P) malic enzyme (ME2) show normal insulin secretion. The mitochondrial NADP malic enzyme (ME3) is very low in pancreatic β-cells, and ME3 was previousl
Autor:
Hui-Young Lee, Richard G. Kibbey, Derek M. Erion, Joao Paulo Camporez, Anila K. Madiraju, Yasmeen Rahimi, Xian-Man Zhang, Michael J. MacDonald, John L. Wood, Gary W. Cline, Gerald I. Shulman, Varman T. Samuel, Michael J. Jurczak, Demetrios T. Braddock, Brett J. Prigaro, Ronald A. Albright, Sanjay Bhanot
Publikováno v:
Nature. 510:542-546
Metformin is considered to be one of the most effective therapeutics for treating type 2 diabetes because it specifically reduces hepatic gluconeogenesis without increasing insulin secretion, inducing weight gain or posing a risk of hypoglycaemia. Fo
Sphingosine kinase 1 knockdown reduces insulin synthesis and secretion in a rat insulinoma cell line
Autor:
Scott W. Stoker, Suzanne G. Laychock, Mindy A. Kendrick, Noah R. Druckenbrod, Lucy D. Mastrandrea, Melissa J. Longacre, Michael J. MacDonald, Noaman Hasan
Publikováno v:
Archives of Biochemistry and Biophysics. 518:23-30
To evaluate the role of sphingosine kinase 1 (SphK1) in insulin secretion, we used stable transfection to knock down the expression of the Sphk1 gene in the rat insulinoma INS-1 832/13 cell line. Cell lines with lowered Sphk1 mRNA expression and SphK
Publikováno v:
Diabetologia. 53:1019-1032
Pancreatic beta cells are specialised endocrine cells that continuously sense the levels of blood sugar and other fuels and, in response, secrete insulin to maintain normal fuel homeostasis. During postprandial periods an elevated level of plasma glu
Publikováno v:
Archives of Biochemistry and Biophysics. 488:100-104
Despite interest in malic enzyme(ME)s in insulin cells, mitochondrial malic enzyme (ME2) has only been studied with estimates of mRNA or with mRNA knockdown. Because an mRNA’s level does not necessarily reflect the level of its cognate enzyme, we d
Autor:
Michael J. MacDonald, Sarawut Jitrapakdee, Mindy A. Kendrick, Thirajit Boonsaen, Melissa J. Longacre, John C. Wallace, Noaman Hasan, Scott W. Stoker
Publikováno v:
Journal of Biological Chemistry. 283:28048-28059
Anaplerosis, the synthesis of citric acid cycle intermediates, by pancreatic beta cell mitochondria has been proposed to be as important for insulin secretion as mitochondrial energy production. However, studies designed to lower the rate of anaplero
Publikováno v:
Biochimica et Biophysica Acta (BBA) - General Subjects. 1780:966-972
We hypothesized that contrasting leucine with its non-metabolizable analog 2-aminobicyclo[2,2,1]heptane-2-carboxylic acid (BCH) might provide new information about metabolic pathways involved in insulin secretion. Both compounds stimulate insulin sec
Publikováno v:
Molecular and Cellular Biochemistry. 313:195-202
Methyl succinate (MS) and alpha-ketoisocaproate (KIC) when applied alone to cultured pancreatic islets or INS-1 832/13 cells do not stimulate insulin release. However, when the two metabolites are combined together they strongly stimulate insulin rel