Zobrazeno 1 - 10
of 27
pro vyhledávání: '"John D. Hildebrandt"'
Autor:
Daniel R. Knapp, Michael D. Wilcox, Kevin L. Schey, Lana A. Cook, John D. Hildebrandt, Troy Nelson, Jane Dingus, Rebecca Ettling
Publikováno v:
Molecular & Cellular Proteomics. 5:671-685
We characterized the variable processing of the G protein gamma subunit isoforms associated with bovine brain G proteins, a primary mediator of cellular communication. Ggamma subunits were isolated from purified brain G proteins and characterized by
Autor:
John D. Hildebrandt, Wanling Yang
Publikováno v:
Cellular Signalling. 18:194-201
Analysis of the genomic sequences, cDNAs and expressed sequence tags (ESTs) in human and mouse for the 12 genes of the gamma subunits of the heterotrimeric G proteins has allowed us to identify the common versus unique elements of the organization an
Publikováno v:
Biochemistry. 37:14651-14658
The structural differences between two major forms of the alpha subunit of the heterotrimeric G protein GO were found to be due to deamidation of either of two Asn residues near the C-terminus of the proteins, in a region involved in receptor recogni
Autor:
John D. Hildebrandt
Publikováno v:
Biochemical Pharmacology. 54:325-339
The heterotrimeric G proteins are extensively involved in the regulation of cells by extracellular signals. The receptors that control them are often the targets of drugs. There are many isoforms of each of the three subunits that make up these prote
Autor:
Joseph P. Pieroni, Gezhi Weng, Jinrong Li, Harel Weinstein, John D. Hildebrandt, Frank Guarnieri, Yibang Chen, Jane Dingus, Ravi Iyengar, Anya Harry
Publikováno v:
Proceedings of the National Academy of Sciences. 94:2711-2714
Receptor activation of heterotrimeric G proteins dissociates G alpha from the G betagamma complex, allowing both to regulate effectors. Little is known about the effector-interaction regions of G betagamma. We had used molecular modeling to dock a pe
Autor:
Jane, Dingus, John D, Hildebrandt
Publikováno v:
Sub-cellular biochemistry. 63
The heterotrimeric GTP-binding proteins (G proteins) are the canonical cellular machinery used with the approximately 700 G protein-coupled receptors (GPCRs) in the human genome to transduce extracellular signals across the plasma membrane. The synth
Publikováno v:
Journal of Biological Chemistry. 277:50223-50225
A ligand-independent activator of heterotrimeric brain G-protein was partially purified from detergent-solubilized extracts of the neuroblastoma-glioma cell hybrid NG108-15. The G-protein activator (NG108-15 G-protein activator (NG-GPA)) increased [(
Publikováno v:
Developmental Biology. 142:129-137
Pertussis toxin ADP-ribosylation and Western blot analysis using G protein-specific antibodies were used to study G protein expression in mouse oocytes, eggs, and early embryos. A pertussis toxin (PT) substrate of about 40 kDa was observed in all sta
Publikováno v:
The Journal of biological chemistry. 282(19)
Between 1 and 2% of proteins coded for in the human genome, including all G protein gamma subunits, are predicted to be prenylated. Subsequently, prenylated proteins are proteolytically cleaved at the C terminus and carboxymethylated. These reactions
Autor:
John D, Hildebrandt
Publikováno v:
Molecular pharmacology. 69(4)
G protein-coupled receptor (GPCR)-Galpha fusion proteins were first characterized more than 10 years ago as a strategy for studying receptor-G protein signaling. A large number of studies have used this approach to characterize receptor coupling to m