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Autor:
K R Porter, J J Wolosewick
Publikováno v:
The Journal of Cell Biology
The cytoplasmic ground substance of cultured cells prepared for high voltage transmission electron microscopy (glutaraldehyde/osmium fixed, alcohol or acetone dehydrated, critical-point dried) consists of slender (3-6 nm Diam) strands--the microtrabe
Autor:
D. E. Chandler, John A. Williams
Publikováno v:
The Journal of Cell Biology
Subcellular distribution of the divalent cation-sensitive probe chlorotetracycline (CTC) was observed by fluorescence microscopy in isolated pancreatic acinar cells, dissociated hepatocytes, rod photoreceptors, and erythrocytes. In each cell type, ar
Publikováno v:
The Journal of Cell Biology
Rhodamine- and fluorescein-labeled gangliosides were used as probes to investigate the distribution, dynamics, and fate of plasma membrane-bound gangliosides on cultured human fibroblasts. When sparse cultures of fibroblasts were incubated with the f
Autor:
Daniel Axelrod
Publikováno v:
The Journal of Cell Biology
A technique for exciting fluorescence exclusively from regions of contact between cultured cells and the substrate is presented. The technique utilizes the evanescent wave of a totally internally reflecting laser beam to excite only those fluorescent
Autor:
Narla Mohandas, Joel Anne Chasis
Publikováno v:
The Journal of Cell Biology
Skeletal proteins play an important role in determining erythrocyte membrane biophysical properties. To study whether membrane deformability and stability are regulated by the same or different skeletal protein interactions, we measured these two pro
Publikováno v:
The Journal of Cell Biology
The intracellularly trapped fluorescent calcium indicator, quin 2, was used not only to monitor changes in cytosolic-free calcium, [Ca2+]i, but also to assess the role of [Ca2+]i in neutrophil function. To increase cytosolic calcium buffering, human
Publikováno v:
The Journal of Cell Biology
The present study has shown that changes in ionic channel currents accompany the phagocytosis of particles by mononuclear phagocytes. The patch-clamp technique in the cell-attached configuration was applied to human monocyte-derived macrophages to me
Autor:
E B Cramer, J I Gallin
Publikováno v:
The Journal of Cell Biology
Potassium pyroantimonate was used to localize sites of bound cations in human neutrophils under conditions of random migration, stimulated random migration (chemokinesis), and directed migration (chemotaxis). The cells were placed in a standard chamb
Autor:
Norman E. Kemp
Publikováno v:
The Journal of Biophysical and Biochemical Cytology
1. In the cytoplasm of oocytes of stage Y0, prior to the appearance of yolk, one observes a few scattered profiles of endoplasmic reticulum and numerous filamentous mitochondria, usually distributed at random but sometimes clustered. As the nuclear m
Publikováno v:
The Journal of Biophysical and Biochemical Cytology
Cells which are infected with measles virus have been known for some time to contain inclusion material that is distinguishable from normal cellular components by application of traditional staining methods and observation in the light microscope. Th