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Publikováno v:
The Journal of Cell Biology
Myosins are actin-based motors that are generally believed to move by amplifying small structural changes in the core motor domain via a lever arm rotation of the light chain binding domain. However, the lack of a quantitative agreement between obser
Autor:
Lunhui Lin, J. H. Morais Cabral, L Stolz, Shirin M. Marfatia, C Hough, P J Bryant, Athar H. Chishti
Publikováno v:
The Journal of Cell Biology
The human homologue (hDIg) of the Drosophila discs-large tumor suppressor (DIg) is a multidomain protein consisting of a carboxyl-terminal guanylate kinase-like domain, an SH3 domain, and three slightly divergent copies of the PDZ (DHR/GLGF) domain.
Publikováno v:
The Journal of Cell Biology
Newly synthesized class II molecules of the major histocompatibility complex must be transported to endosomal compartments where antigens are processed for presentation to class II-restricted T cells. The invariant chain (Ii), which assembles with ne
Autor:
Pierre A. Coulombe, Elaine Fuchs
Publikováno v:
The Journal of Cell Biology
Because of extraordinarily tight coiled-coil associations of type I and type II keratins, the composition and structure of keratin subunits has been difficult to determine. We report here the use of novel genetic and biochemical methods to explore th
Autor:
G W Zieve, R J Feeney
Publikováno v:
The Journal of Cell Biology
The snRNP particles include a set of common core snRNP proteins and snRNP specific proteins. In rodent cells the common core proteins are the B, D, D', E, F and G proteins in a suggested stoichiometry of B2D'2D2EFG. The additional U1- and U2-specific
Publikováno v:
The Journal of Cell Biology
The nuclear matrix is concealed by a much larger mass of chromatin, which can be removed selectively by digesting nuclei with DNase I followed by elution of chromatin with 0.25 M ammonium sulfate. This mild procedure removes chromatin almost complete
Publikováno v:
The Journal of Cell Biology
Plasma fibronectin binds saturably and reversibly to substrate-attached fibroblasts and is subsequently incorporated into the extracellular matrix (McKeown-Longo, P.J., and D. F. Mosher, 1983, J. Cell Biol., 97:466-472). We examined whether fragments
Publikováno v:
The Journal of Cell Biology
The structure of native and progressively reduced human factor VIII/von Willebrand factor (FVIII/vWF) was examined by electron microscopy and SDS gel electrophoresis and then correlated with its biological activities. Highly resolved electron microgr
Autor:
J S Morrow, V T Marchesi
Publikováno v:
The Journal of Cell Biology
Purified human erythrocyte spectrin is able to form large oligomeric species without the collaboration of any other proteins. This reversible self-assembly process is both temperature and concentration dependent and seems to be mediated by the same k
Autor:
Richard Niederman, Thomas D. Pollard
Publikováno v:
The Journal of Cell Biology
We have used electron microscopy and solubility measurements to investigate the assembly and structure of purified human platelet myosin and myosin rod into filaments. In buffers with ionic strengths of less than 0.3 M, platelet myosin forms filament