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Autor:
Michele Bastiani, John F. Hancock, Vikas A. Tillu, James Rae, Yong Zhou, Hong Liang, Adekunle T. Bademosi, B. Collins, Nicholas Ariotti, Shern Tee, Robert G. Parton, Frederic A. Meunier
Publikováno v:
The Journal of Cell Biology
We integrated quantitative nanoscale lipid mapping with molecular dynamic simulations to define the lipid profile within caveolae. Caveolin-1 and cavin1 individually sort distinct plasma membrane lipids. Intact caveolae containing both caveolin-1 and
Autor:
Andrew P. Kowalczyk, William F. Dean, Alexa L. Mattheyses, Tara M. Urner, Reena R. Beggs, Emily I. Bartle, Tejeshwar C. Rao
Publikováno v:
The Journal of Cell Biology
Desmosomes regulate intercellular adhesion, which controls tissue strength and plasticity. Bartle et al. show that desmosome adhesive strength is modulated through protein exchange in and out of desmosomes, not cadherin order. This exchange is tuned
Autor:
John M. Heddleston, Eric Wait, Maddy Parsons, Karin Pfisterer, Campbell D. Lawson, Susan Cox, James A. Levitt, Richard J. Marsh, Simon Ameer-Beg
Publikováno v:
The Journal of Cell Biology
Pfisterer, K, Levitt, J, Lawson, C, Marsh, R, Heddleston, J, Wait, E, Ameer-Beg, S, Cox, S & Parsons, M 2020, ' FMNL2 regulates dynamics of fascin in filopodia ', Journal of Cell Biology, vol. 219, no. 5, e201906111 . https://doi.org/10.1083/jcb.201906111
Pfisterer, K, Levitt, J, Lawson, C, Marsh, R, Heddleston, J, Wait, E, Ameer-Beg, S, Cox, S & Parsons, M 2020, ' FMNL2 regulates dynamics of fascin in filopodia ', Journal of Cell Biology, vol. 219, no. 5, e201906111 . https://doi.org/10.1083/jcb.201906111
Fascin is highly dynamic within filopodia and undergoes rapid changes in phosphorylation that dictate localized F-actin bundling activity. Pfisterer et al. show that fascin binds directly to FMNL2, and this controls fascin positioning and entry into
Publikováno v:
The Journal of Cell Biology
J. Cell Biol. 217, 1643-1649 (2018)
J. Cell Biol. 217, 1643-1649 (2018)
Using single-particle electron microscopy of the human insulin receptor reconstituted into nanosdiscs, Gutmann et al. show that ligand binding induces a conformational rearrangement in the receptor ectodomain that results in the dimerization of the t
Autor:
Jing Li, Timothy A. Springer
Publikováno v:
The Journal of Cell Biology
Li and Springer demonstrate differences between integrins α4β1 and α5β1 in intrinsic affinities and relative free energies of three conformational states. Integrin conformational equilibria are both subunit and cell type specific. The energy land
Publikováno v:
The Journal of Cell Biology
Kapinos et al. show that nuclear pore complex permeability and cargo release functionalities are concomitantly regulated by karyopherin occupancy and turnover in a systematic continuum. This highlights increasingly important roles for the soluble nuc
Autor:
Annett Neuner, Elmar Schiebel, Robert A. Arkowitz, Takumi Chinen, Dirk Flemming, Tien-chen Lin, Ursula Jäkle, Peng Liu
Publikováno v:
The Journal of Cell Biology
Cells use γ-tubulin complex to nucleate microtubules. The assembly of active microtubule nucleator is spatially and temporally regulated through the cell cycle. Lin et al. show that the protein Mzt1/MOZART1 and γ-tubulin complex receptors directly
Autor:
Caiting Yu, Xiaofang Huang, Zhiyong Lou, Zihe Rao, Xin Zhou, Liming Yan, Yuanbo Qi, Xiangyang Guo, Junjie Hu, Xiaoyu Hu
Publikováno v:
The Journal of Cell Biology
Mitofusin 1 (MFN1) mediates mitochondrial fusion, but the mechanisms involved are unclear. Qi et al. present the crystal structures of a minimal GTPase domain of human MFN1, which suggest that MFN1 tethers apposing membranes through nucleotide-depend
Publikováno v:
The Journal of General Physiology
A novel method is presented to measure short distances in cell plasma membranes using transition metal ion FRET with metal ions bound to introduced sites in the membrane.
Biological membranes are complex assemblies of lipids and proteins that se
Biological membranes are complex assemblies of lipids and proteins that se
Autor:
Helder Maiato, Hugo Girão, Sandra Macedo-Ribeiro, Zaira Garcia, Ikuko Hayashi, Jorge E. Azevedo, Alexandra Silva, Tatiana Moutinho-Santos, Tony A. Rodrigues, Ana C. Figueiredo, Naoyuki Okada
Publikováno v:
The Journal of Cell Biology
Girão et al. use structure-guided functional mutants of CLASP2 to show that recognition of growing microtubule plus-ends through EB–protein interaction and the ability to associate with curved microtubule protofilaments through TOG2 and TOG3 domai