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Publikováno v:
Proceedings of the National Academy of Sciences. 100:6394-6397
We have successfully used mutagenesis to engineer Taxol (paclitaxel) binding activity in Saccharomyces cerevisiae tubulin. Taxol, a successful antitumor agent, acts by promoting tubulin assembly and stabilizing microtubules. Several structurally dive
The anticancer drug–DNA complex: Femtosecond primary dynamics for anthracycline antibiotics function
Publikováno v:
Proceedings of the National Academy of Sciences. 98:14212-14217
The anthracycline–DNA complex, which is a potent agent for cancer chemotherapy, has a unique intercalating molecular structure with preference to the GC bases of DNA, as shown by Rich's group in studies of single-crystal x-ray diffraction. Understa
Publikováno v:
Proceedings of the National Academy of Sciences. 96:840-845
Excess 17β-estradiol (E 2 ), the most potent of human estrogens, is known to act as a stimulus for the growth of breast tumors. Human estrogenic 17β-hydroxysteroid dehydrogenase type 1 (17β-HSD1), which catalyzes the reduction of inactive estrone
Publikováno v:
Proceedings of the National Academy of Sciences. 90:11598-11602
We have previously shown that rat brain tubulin, a heterodimer consisting of an alpha and beta monomer, can be covalently labeled with [3H]colchicine by near UV irradiation. Most of the label appears in beta-tubulin. We show here that beta-tubulin ca
Publikováno v:
Proceedings of the National Academy of Sciences. 88:10601-10604
In addition to generating polymeric products from human fibrinogen, human erythrocyte transglutaminase (protein-glutamine:amine gamma-glutamyltransferase, EC 2.3.2.13) was shown to catalyze the intramolecular reaction of crosslinking two of the const
Autor:
László Muszbek, Koloman Laki
Publikováno v:
Proceedings of the National Academy of Sciences. 71:2208-2211
Under certain conditions actin can be split by thrombin. Actin prepared in the presence of excess Ca ++ was found to be resistant to thrombin. However, if actin was purified without added Ca ++ , both G- and F- actin underwent thrombic digestion, alt
Publikováno v:
Proceedings of the National Academy of Sciences. 80:2467-2471
We propose a primary structure for the catalytic subunit of aspartate transcarbamoylase (aspartate carbamoyltransferase; carbamoylphosphate: L-aspartate carbamoyltransferase, EC 2.1.3.2) from Escherichia coli based on amino acid sequences of fragment
Autor:
David W. Rowe, Karen E. Peterson, Joseph C. C. Fu, Robert C. Siegel, Karen A. Holbrook, Yu-Hua Chang, Lynne T. Smith, Peter H. Byers
Publikováno v:
Proceedings of the National Academy of Sciences. 78:7745-7749
Cells in culture from a woman with a variety of the Marfan syndrome produce two species of the alpha 2 chains of type I collagen. One alpha 2 chain appears normal; the abnormal chain has a higher apparent molecular weight than normal and migrates mor
Purification and affinity labeling of dihydropyridine receptor from rabbit skeletal muscle membranes
Publikováno v:
Proceedings of the National Academy of Sciences. 85:2969-2973
Undegraded dihydropyridine (DHP)-receptor (putatively a voltage-gated Ca2+ channel) has been purified as a 340-kDa protein complex to approximately 80% homogeneity (2.4 nmol of DHP-receptor per mg of protein) from rabbit skeletal muscle by a rapid pu
Publikováno v:
Proceedings of the National Academy of Sciences. 79:4618-4622
Carboxypeptidase N (kininase I, arginine carboxypeptidase; EC 3.4.17.3) cleaves COOH-terminal basic amino acids of kinins, anaphylatoxins, and other peptides. The tetrameric enzyme of Mr 280,000 was purified from human plasma by ion-exchange and argi