Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Joan J. Guinovart"'
Dynamic profiling of the glucose metabolic network in fasted rat hepatocytes using [1,2-13C2]glucose
Autor:
Silvia Marin, Shu Lim, Josep J. Centelles, W. N. Paul Lee, Joan J. Guinovart, Sara Bassilian, Josep M. Fernández-Novell, Laszlo G. Boros, Marta Cascante
Publikováno v:
Biochemical Journal. 381:287-294
Recent studies in metabolic profiling have underscored the importance of the concept of a metabolic network of pathways with special functional characteristics that differ from those of simple reaction sequences. The characterization of metabolic fun
Autor:
Mar GARCÍA-ROCHA, Angela ROCA, Núria de la IGLESIA, Otto BABA, Josep M. FERNÁNDEZ-NOVELL, Juan C. FERRER, Joan J. GUINOVART
Publikováno v:
Biochemical Journal. 357:17-24
Changes in the intracellular distribution of liver glycogen synthase (GS) might constitute a new regulatory mechanism for the activity of this enzyme at cellular level. Our previous studies indicated that incubation of isolated hepatocytes with gluco
Publikováno v:
Biochemical Journal. 351:811-816
We have used recombinant adenoviruses (AdCMV-RLGS and AdCMV-GK) to overexpress the liver isoforms of glycogen synthase (GS) and glucokinase (GK) in primary cultured rat hepatocytes. Glucose activated overexpressed GS in a dose-dependent manner and ca
Autor:
David Bellido, Joan J. Guinovart, Joan Seoane, Senén Vilaró, Josep M. Fernández-Novell, Anna M. Gómez-Foix
Publikováno v:
Biochemical Society Transactions. 25:157-160
Publikováno v:
Biochemical Journal. 304:449-453
The ability of amylin to impair hepatic insulin action is controversial. We have found that the effect of amylin in primary cultured hepatocytes is strongly dependent on the culture conditions. Only in hepatocytes preincubated in the presence of feta
Publikováno v:
Scopus-Elsevier
Incubation of rat hepatocytes with glucose induces the translocation of glycogen synthase from soluble fractions to fractions which sediment at 10,000 g. Incubation of the cells with fructose, galactose, 2-deoxyglucose or 5-thioglucose, which activat
Publikováno v:
Biochemical Journal. 281:443-448
Incubation of rat hepatocytes with glucose results in a decrease in the amount of glycogen synthase activity found in supernatants obtained after centrifugation of cell homogenates at 9200 g. The enzymic activity was quantitatively recovered in the s
Autor:
Joan J. Guinovart
Publikováno v:
The Biochemist. 26:45-46
Autor:
Mar García-Rocha, Joan C. Ferrer, Josep M. Fernández-Novell, Susanna Baqué, Joan J. Guinovart
Publikováno v:
Biochemical Society Transactions. 28:A99-A99
Publikováno v:
Biochemical Journal. 261:93-97
Prostaglandin E2 (PGE2) and prostaglandin F2 alpha (PGF2 alpha) inactivated glycogen synthase and activated glycogen phosphorylase in rat hepatocytes in a dose- and time-dependent manner. These effects were dependent on the presence of Ca2+ in the in