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Publikováno v:
International Immunology. 27:633-645
Polyclonal antibodies hydrolyzing myelin basic protein (MBP) can play an important role in the pathogenesis of multiple sclerosis and systemic lupus erythematosus (SLE). An immunoglobulin light chain phagemid library derived from peripheral blood lym
Publikováno v:
Journal of Insect Science
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Roles of salivary proteases in the extra-oral digestion of the predatory bug, Andrallus spinidens Fabricius (Hemiptera: Pentatomidae) were studied by using 2% azocasein as a general substrate and specific protease substrates, as well as synthetic and
Autor:
Koichi Mori, Tetsuo Toraya, Hirohisa Sakamoto, Satoshi Kawaguchi, Yuka Nakanishi, Naoki Hieda, Mamoru Yamanishi, Keita Akita, Nobuyuki Baba
Publikováno v:
Journal of Biochemistry. 147:83-93
The methods of homologous high-level expression and simple large-scale purification for coenzyme B(12)-dependent ethanolamine ammonia-lyase of Escherichia coli were developed. The eutB and eutC genes in the eut operon encoded the large and small subu
Autor:
Dolores de Arriaga, Javier Rúa, Sergio de Cima, Pilar del Valle, Félix Busto, Alberto Baroja-Mazo
Publikováno v:
Journal of Biochemistry. 142:247-255
The stability of acetyl-CoA synthetases (ACS1 and ACS2) from P. blakesleeanus against temperature, urea and trypsin was studied and compared. Thermal inactivation of ACS1 was biphasic, while that of ACS2 was monophasic. The thermodynamic parameters c
Autor:
Akihiro Kondo, Xiangchun Wang, Ikunoshin Kato, Yoko Mizuno-Horikawa, Nobuto Koyama, Wenzhe Li, Takatoshi Nakagawa, Naoyuki Taniguchi, Koichi Honke, Eiji Miyoshi, Jianguo Gu, Jinhua Jin
Publikováno v:
Glycobiology. 16:1007-1019
Alpha1,6-fucosyltransferase (Fut8) plays important roles in physiological and pathological conditions. Fut8-deficient (Fut8-/-) mice exhibit growth retardation, earlier postnatal death, and emphysema-like phenotype. To investigate the underlying mole
Publikováno v:
FEMS Microbiology Letters. 154:145-150
We report here that Vibrio anguillarum possesses a non-inducible active transport system which can efficiently supply iron to the cell from ferric citrate, independently of the siderophore-based mechanisms. The strains tested were able to grow in CM9
Autor:
Agnieszka Banbula, Charu L. Bohra, Wayne F. Beyer, George J. Cianciolo, Salvatore V. Pizzo, Lara S. Chang
Publikováno v:
The Journal of Biochemistry. 138:527-537
We have characterized native and activated forms of rabbit alpha1M and compared them to rabbit and human alpha2M. Similar to human alpha2M, rabbit alpha1M is a tetramer associated via disulfide bonds and non-covalent interactions that exhibits autoly
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 68:300-305
A method is proposed to produce a foodstuff that inhibits allergen absorption through the intestinal tract. Defatted black sesame (Sesamum indicum) seeds as a starting material were hydrolyzed with a crude preparation of trypsin at 40 degrees C and p
Publikováno v:
Journal of Biochemistry. 134:31-42
Trypsin and papain proteinase inhibitors have been identified and purified from aqueous extracts of apple seeds (Malus domestica). Superdex G75 gel filtration chromatography identified a higher molecular weight (HMW) papain inhibitory fraction (22-26
Publikováno v:
Journal of Biochemistry. 133:231-237
The physiological interaction between glycyrrhizin (GL) and serum complement C3, and the inhibitory effects of GL, glycyrrhetinic acid (GA), and a GA derivative (oGA) on the phosphorylation of C3 by casein kinase 2 (CK-2), were investigated in vitro.