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Publikováno v:
Journal of Applied Microbiology. 124:667-681
Aims β-lactamase inhibitor resistance (BLIR) among the uropathogenic Escherichia coli (UPEC) minimizes treatment options. This study aimed to identify inhibitor-resistant TEM (IRT) β-lactamase that impart BLIR phenotype and explore non-β-lactams a
Autor:
Per Jr Greisen, Sergey Ovchinnikov, Yacov Ashani, Sarel J. Fleishman, Joel L. Sussman, Moshe Goldsmith, Dan S. Tawfik, Halim Jubran, David Baker, Adi Goldenzweig, Nidhi Aggarwal, Haim Leader
Publikováno v:
Protein Engineering, Design and Selection. 30:333-345
Improving an enzyme's initially low catalytic efficiency with a new target substrate by an order of magnitude or two may require only a few rounds of mutagenesis and screening or selection. However, subsequent rounds of optimization tend to yield dec
Autor:
Robert J. Linhardt, Payel Datta, Jeffrey D. Esko, Bo Yang, Jong Youn Baik, Trent R. Gemmill, Guoyun Li, Charles A. Glass, Susan T. Sharfstein, Leyla Gasimli
Publikováno v:
Glycobiology, vol 24, iss 3
Heparin (HP), an important anticoagulant polysaccharide, is produced in a complex biosynthetic pathway in connective tissue-type mast cells. Both the structure and size of HP are critical factors determining the anticoagulation activity. A murine mas
Autor:
Enrico A. Stura, Frédéric Ducancel, Alexandra Savatier, Janie Dassa, Narciso Costa, Guillaume L'Hostis, Laurent Mesta, Bruno H. Muller, Jean-Claude Boulain, Alain Troesch
Publikováno v:
Protein Engineering Design and Selection. 26:725-734
A single strategy to select RNA polymerase from bacteriophage T7 (T7 RNAP) mutants in Escherichia coli with enhanced thermostability or enzymatic activity is described. T7 RNAP has the ability to specifically transcribe genes under control of T7 phag
Autor:
Mahvash Khodabandeh, Zarrin Minuchehr, Fatemeh Tabandeh, Mojtaba Samoudi, N Omid Yeganeh, S W Harcum
Publikováno v:
Protein Engineering, Design & Selection.
Recombinant human interferon-β (rhIFN-β) used clinically has lower efficacy than expected due to protein instabilities such as aggregation. Increasing molecular stability, glycoengineering has been used to improve clinical efficacy for a number of
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 75:804-807
We made two series of Gateway binary vectors, pGWBs and R4pGWBs, possessing a UDP-N-acetylglucosamine: dolichol phosphate N-acetylglucosamine-1-P transferase (GPT) gene driven by the nopaline synthase promoter (Pnos) as a tunicamycin resistance marke
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 75:723-731
Cordycepin, a pure compound of Cordyceps sinensis (CS), is known as an adenosine analog. We have found that CS stimulated Leydig cell steroidogenesis. Here we investigated the in vivo and in vitro effects of cordycepin in primary mouse Leydig cell st
Autor:
Dae Young Kwon, Choong Hwan Lee, Young Suk Kim, Jung Nam Choi, Hyung-Kyoon Choi, Gun Hee Son, Jiyoung Kim, Daejung Kang
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 75:732-739
Liquid chromatography mass spectrometry and multivariate analysis were employed to investigate the correlation between fermentation time-dependent metabolite changes in cheonggukjang, a traditional fermented soybean product, and changes in its antiox
Autor:
Kozo Ochi, Takashi Inaoka
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 75:618-623
Microorganisms possess the ability to produce a variety of commercially important secondary metabolites such as antibiotics. Although it becomes harder and harder to discover useful new compounds, microorganisms still have the potential to produce un
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 75:7-12
The uptake activity ratio for AMP, ADP, and ATP in mutant (T-1) cells of Escherichia coli W, deficient in de novo purine biosynthesis at a point between IMP and 5-aminoimidazole-4-carboxiamide-1-β-D-ribofuranoside (AICAR), was 1:0.43:0.19. This rati