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pro vyhledávání: '"Shipley, Janet"'
Figure S7. Effect of MYCN knockdown on MYCNOS-02 transcript expression in RMS and NB. (A) qRT-PCR and (B) Western blot for MYCN, and (C) qRT-PCR for MYCNOS-02 after treatment with three pooled MYCN siRNAs for 72 h in RMS-01. (D)-(F) in KELLY as for (
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::317ade83817011ce44c2fcc76d7081e5
Figure S6. Effect of MYCNOS-02 knockdown on MYCN transcript and protein expression in RMS and NB. qRT-PCR detecting MYCNOS-02 transcript level after MYCNOS-02 knockdown with three siRNAs in (A) RMS-01 and (D) KELLY. Corresponding MYCN transcript leve
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::07fc92532bc5eaa302aa03904db9b92f
Figure S4. Effect of MYCNOS-01 overexpression on MYCN transcript and protein expression. qRT-PCR detecting MYCNOS-01 transcript level after 72 h transient transfection with two MYCNOS-01 overexpressing vectors or empty vector control in (A) RMS-01 an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c5ba38312f6f3b51043200f8b7c5af6
Figure S9. Knockdown of MYCNOS-01 does not induce apoptosis. (A) Caspase 3/7 signalling intensity in RMS-01 after transfection with three MYCNOS-01 siRNAs or three pooled MYCN siRNAs for 96 h relative to cell viability and normalised to NT siRNA. (B)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42358bed6febb8c5a34fb828967c09fa
Figure S3. Effect of MYCNOS-01 knockdown on MYCN protein expression in RMS and NB measured by immunofluorescence. (A) Immunofluorescence staining of MYCN (red) with DAPI shown in blue after MYCNOS-01 knockdown for 72 h with three siRNAs in RMS-01. (B
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::360275d2258780c5004f9ce2264299f3
Figure S8. Knockdown of MYCNOS-02 inhibits cell viability. (Ai) MTS assay showing cell viability of RMS-01 over 96 h after transfection with three MYCNOS-02 siRNAs relative to NT siRNA. (Bi) KELLY as in (Ai). Corresponding qRT-PCR of MYCNOS-02 (ii) a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::237cc4aeb54357b9cc2b8c79ed3b8a07
Figure S1. Relative genomic positions of MYCNOS-01, MYCNOS-02 and MYCN and protein-coding potential of MYCNOS-01. (A) Diagram illustrating relative positions of MYCNOS-01, MYCNOS-02 and MYCN exons. Modified from UCSC Genome Browser. (B) Predicted seq
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::495cb047785aac8eb38791aa5197f234
Figure S11. Knockdown of MYCNOS-01 does not affect cell cycle progression. (A) Flow cytometry 96 h post-transfection with three MYCNOS-01 siRNAs or three pooled MYCN siRNAs in RMS-01, (B) KELLY, (C) RH30, and (D) SY5Y. Graphs show an average of three
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad965bbbfa222524e25fd61c08e6e48a
Figure S10. Knockdown of MYCNOS-02 does induce apoptosis. (A) Caspase 3/7 signalling intensity in RMS-01 after transfection with three MYCNOS-02 siRNAs for 96 h relative to cell viability and normalised to NT siRNA. (B) Western blot of total and clea
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52d48d861f8446eb5a63f9053998f32d