Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Timothy J. Mitchison"'
Publikováno v:
Cell Reports, Vol 8, Iss 6, Pp 1808-1818 (2014)
Poly(ADP-ribose) polymerases (PARPs) catalyze poly(ADP-ribose) addition onto proteins, an important posttranslational modification involved in transcription, DNA damage repair, and stem cell identity. Previous studies established the activation of PA
Externí odkaz:
https://doaj.org/article/9a74eeeafbc34a3989a4ce63c759f033
Undiluted cytoplasmic extract prepared from unfertilized Xenopus laevis eggs by low-speed centrifugation (CSF extracts) is useful for reconstitution of egg microtubule dynamics and meiosis-II spindle organization, but it suffers limitations for bioch
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ab57d77ec7b8a0c5ba1ec8fa00ca5d6c
https://doi.org/10.1016/b978-0-12-397924-7.00023-6
https://doi.org/10.1016/b978-0-12-397924-7.00023-6
We report optimized methods for preparing Xenopus egg extracts without cytochalasin D, that we term "actin-intact egg extract." These are undiluted egg cytoplasm that contains abundant organelles, and glycogen which supplies energy, and represents th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0446c877b0ff10a4c238e156dc3c3bc5
https://doi.org/10.1016/b978-0-12-397924-7.00022-4
https://doi.org/10.1016/b978-0-12-397924-7.00022-4
Autor:
Sophie Dumont, Timothy J. Mitchison
Mitosis, the process by which the genome is segregated equally during cell division, is an intrinsically mechanical process. Although we now have a nearly complete list of molecules essential to cell division, how these molecules generate the emergen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8a99487012cf8fa43cdcf19828f45c60
https://doi.org/10.1016/b978-0-12-374920-8.00419-7
https://doi.org/10.1016/b978-0-12-374920-8.00419-7
The large and transparent cells of cleavage-stage zebrafish embryos provide unique opportunities to study cell division and cytoskeletal dynamics in very large animal cells. Here, we summarize recent progress, from our laboratories and others, on liv
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1546dcfcebb8e7313641626a1bbea922
https://doi.org/10.1016/b978-0-12-387036-0.00001-3
https://doi.org/10.1016/b978-0-12-387036-0.00001-3
Publisher Summary This chapter discusses fluorescence photoactivation as an alternative technology for probing cytoskeleton dynamics. In this approach, the protein is tagged with a caged fluorochrome. This probe molecule is nonfluorescent until illum
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6085183fc174946d71a647d9fa8e84ff
https://doi.org/10.1016/s0076-6879(98)91007-2
https://doi.org/10.1016/s0076-6879(98)91007-2