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Publikováno v:
Reproduction, Fertility and Development. 23:837
The present study aimed to develop a set of sensitive assays to evaluate the presence of different isoforms, the activity degree, and the immunolocalisation of proacrosin–acrosin in sexually mature boars. The goal was to determine the proacrosin–
Publikováno v:
Reproduction, Fertility and Development. 23:603
Sperm–oviduct interactions in Bennett’s wallaby (Macropus rufogriseus rufogriseus) were investigated using in vitro cocultures of cauda epididymal spermatozoa and oviducal epithelial cells. Kidney epithelial cells were used as non-reproductive co
Autor:
Csaba Pribenszky, Maurício Machaim Franco, E. S. Caixeta, M. Molnár, E. Siqueira Filho, Rodolfo Rumpf, Andrea Harnos, Ákos Horváth
Publikováno v:
Reproduction, Fertility and Development. 23:585
Sublethal stress treatment has been reported to enhance gametes’ performance in subsequent procedures, such as cryopreservation. The aim of the present study was to evaluate the effect of different equilibration times between the termination of a s
Autor:
C. Steilmann, Marek Bartkuhn, Agnieszka Paradowska, Wolfgang Weidner, Klaus Steger, Martin Bergmann, Sabine Kliesch, Hans-Christian Schuppe, Markus Vieweg
Publikováno v:
Reproduction, Fertility and Development. 23:997
During spermatogenesis, approximately 85% of histones are replaced by protamines. The remaining histones have been proposed to carry essential marks for the establishment of epigenetic information in the offspring. The aim of the present study was to
Autor:
Huai-Liang Feng, Shuiying Ma, Keliang Wu, Hui-Jun Yang, Yuan Li, Wanxia Zhong, Li Che, Mei Li, Zi-Jiang Chen
Publikováno v:
Reproduction, Fertility and Development. 23:433
The objective of the present study was to evaluate the developmental potential and clinical application value of metaphase I (MI) oocytes obtained from stimulated intracytoplasmic sperm injection (ICSI) cycles. ICSI was performed on MI oocytes immedi
Publikováno v:
Reproduction, Fertility and Development. 23:534
The aim of this study was to compare gene silencing in bovine zygotes when small interfering RNAs (siRNAs) were introduced into bovine zygotes by microinjection or lipid-based transfection. In Experiment 1, E-cadherin siRNA was injected at 100 or 375
Publikováno v:
Reproduction, Fertility and Development. 22:733
Identification and enrichment of spermatogonial stem cells (SSCs) are critical steps in testis germ cell transplantation. The present study shows that expression of the protein promyelocytic leukaemia zinc-finger (PLZF) does not occur in all cells, o
Autor:
Ali Honaramooz, Yanfei Yang
Publikováno v:
Reproduction, Fertility and Development. 22:523
The effects of medium and hypothermic temperatures on testis cells were investigated to develop a strategy for their short-term preservation. Testes from 1-week-old piglets were enzymatically dissociated for cell isolation. In Experiment 1, testis ce
Autor:
D Rath, Patrick Lonergan, D.A.F. Villagómez, Angus D. Macaulay, M. Garcia-Herreros, W.A. King, T.F. Carter
Publikováno v:
Reproduction, Fertility and Development. 22:1272
The aim of the present study was to examine the incidence of chromosomal abnormalities in bovine blastocysts produced by IVF with unsorted, X-sorted or Y-sorted spermatozoa. In Experiment 1, individual blastocysts were processed to examine the incide
Autor:
Clara Slade Oliveira, T. A. D. Tetzner, Marina Ragagnin de Lima, Naiara Zoccal Saraiva, Marcela Maria de Souza, Joaquim Mansano Garcia
Publikováno v:
Reproduction, Fertility and Development. 22:1041
Trichostatin A (TSA) induces histone hyperacetylation by inhibiting histone deacetylases and consequently increasing gene expression. The hypothesis was that TSA supplementation during the in vitro culture (IVC) of bovine embryos would increase the b