Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Yoshiyuki Takahashi"'
Publikováno v:
Theriogenology. 80:793-799
Bovine ovaries offer a large pool of oocytes that could be used for in vitro production of embryos of genetically valuable animals. The effects of in vitro growth (IVG) culture duration (10, 12, and 14 days) on the viability and growth of bovine oocy
Autor:
Akira Minamihashi, Ken Sawai, Hiroki Hirayama, Seiji Katagiri, Soichi Kageyama, Sadao Onoe, Keiko Watanabe, Sigenori Matsuzaki, Hidenari Yamashina, Tsugunori Notomi, Satoru Moriyasu, Yoshiyuki Takahashi, Keiko Toen
Publikováno v:
Theriogenology. 62:887-896
Loop-mediated isothermal amplification (LAMP) is a novel DNA amplification method that amplifies a target sequence specifically under isothermal conditions. The product of LAMP is detected by the turbidity of the reaction mixture without electrophore
Publikováno v:
Theriogenology. 61:15-23
We determined the efficacy of a microdrop vitrification procedure for cryopreservation of bovine oocytes, using vitrified oocytes as cytoplasts for intraspecies and intergeneric somatic cell nucleus transfer (NT). In vitro matured bovine MII oocytes
Publikováno v:
Theriogenology. 48:605-616
To study the effects of insulin and insulin-like growth factor-I (IGF-I) on the development of bovine embryos, fertilized bovine embryos in vitro were cultured in a chemically defined, protein-free medium: modified synthetic oviduct fluid (mSOF) supp
Publikováno v:
Theriogenology. 69(2)
This study was conducted to improve in vitro production of embryos from domestic cats using TCM-199 as an IVM medium. The time sequence of nuclear maturation and the optimal timing of in vitro insemination were examined. Most oocytes were at the germ
Rapid sexing of water buffalo (Bubalus bubalis) embryos using loop-mediated isothermal amplification
Autor:
Keiko Watanabe, Akira Minamihashi, Soichi Kageyama, Shinichi Kojiya, Tsugunori Notomi, Satoru Moriyasu, Ken Sawai, Sadao Onoe, Hiroki Hirayama, Yoshiyuki Takahashi
Publikováno v:
Theriogenology. 66(5)
Loop-mediated isothermal amplification (LAMP) is a novel DNA amplification method that amplifies a target sequence specifically under isothermal conditions. The objective of this study was to identify a Y chromosome-specific sequence in water buffalo
Autor:
Seiji Katagiri, Yoshiyuki Takahashi
Publikováno v:
Theriogenology. 62(1-2)
The objective of the present study was to determine if abnormalities in the cyclic changes of endometrial EGF concentrations can be a diagnostic tool for repeat breeder cows. First, the profile of EGF concentrations during the estrous cycle was deter
Publikováno v:
Theriogenology. 51(3)
To improve the enucleation rate in newly matured bovine oocytes, we investigated the position of cytoplasmic chromatin in relation to the polar body and the consequent enucleation efficiency before and after sequential activation with calcium ionopho
Autor:
Melih Aksoy, Yoshiyuki Takahashi, Adil Salim Elsheikh, Hozumi Tanaka, Hiroshi Kanagawa, Mitsugu Hishinuma
Publikováno v:
Theriogenology. 51(4)
Effects of the embryo retrieval stages and addition of glutathione (GSH) on post-thaw development of mouse morula were evaluated in 2 consecutive experiments. In the first experiment, 1-, 2-, 3- to 4- and 5- to 8-cell stage embryos were collected and
Publikováno v:
Theriogenology. 45(6)
Mouse demi-embryos that developed from bisected morulae were transferred to recipients. The eu-blastocysts (distinct inner cell mass and well-developed trophectoderm) contained cells equal to 51% of the controls that developed from zona-free morulae.