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Publikováno v:
The Journal of cell biology. 221(1)
The key endosomal regulators Rab5, EEA1, and APPL1 are frequently applied in fluorescence microscopy to mark early endosomes, whereas Rab7 is used as a marker for late endosomes and lysosomes. However, endogenous levels of these proteins localize poo
Autor:
Ni Tang, Marianna Leonzino, Michael G. Hanna, Pietro De Camilli, Hongying Shen, Andrés Guillén-Samander
Publikováno v:
The Journal of Cell Biology
Mitochondria, which are excluded from the secretory pathway, depend on lipid transport proteins for their lipid supply from the ER, where most lipids are synthesized. In yeast, the outer mitochondrial membrane GTPase Gem1 is an accessory factor of ER
Autor:
Magdalena Prechova, Zuzana Adamova, Anna-Lena Schweizer, Miloslava Maninova, Andreas Bauer, Delf Kah, Samuel M. Meier-Menches, Gerhard Wiche, Ben Fabry, Martin Gregor
Publikováno v:
The Journal of cell biology. 221(3)
The coordinated interplay of cytoskeletal networks critically determines tissue biomechanics and structural integrity. Here, we show that plectin, a major intermediate filament-based cytolinker protein, orchestrates cortical cytoskeletal networks in
CRISPR screens for lipid regulators reveal a role for ER-bound SNX13 in lysosomal cholesterol export
Publikováno v:
The Journal of cell biology. 221(2)
We report here two genome-wide CRISPR screens performed to identify genes that, when knocked out, alter levels of lysosomal cholesterol or bis(monoacylglycero)phosphate. In addition, these screens were also performed under conditions of NPC1 inhibiti
Autor:
Boyi Gan
Publikováno v:
The Journal of cell biology. 220(9)
Ferroptosis is a form of iron-dependent regulated cell death driven by uncontrolled lipid peroxidation. Mitochondria are double-membrane organelles that have essential roles in energy production, cellular metabolism, and cell death regulation. Howeve
Autor:
Katherine E. Bohnsack, Ákos Farkas
Publikováno v:
The Journal of Cell Biology
Farkas and Bohnsack describe advances in understanding targeting of tail-anchored (TA) proteins, including their insertion into the ER, how nascent TA proteins are selectively captured at the ribosome, and recently discovered quality control pathways
Autor:
Yiru Cheng, Lei Yin, Jianguo Chen, Pengli Zheng, Donghui Zhang, Chuyi Hou, Yingchun Hu, Junlin Teng, Qingzhou Chen, Peiyuan Chai
Publikováno v:
The Journal of Cell Biology
ER-mitochondria contacts respond to cellular stress. Chai et al. reveal that in response to hypoxia, USP19, an ER-resident deubiquitinase, accumulates at ER-mitochondria contact sites where USP19 deubiquitinates and stabilizes FUNDC1, thereby promoti
Autor:
Chad Liu, Nalin Ratnayeke, Jeannine Gerhardt, Marielle S. Köberlin, Madhura Deshpande, Tobias Meyer, Yilin Fan
Publikováno v:
The Journal of Cell Biology
Fan et al. show that replisome disassembly during S phase is required for efficient DNA replication by recycling essential replisome components between chromatin-bound and soluble compartments.
After two converging DNA replication forks meet, ac
After two converging DNA replication forks meet, ac
Autor:
Michael J Newbold, Anurag Paul, Jun-Lin Guan, Megan E Bischoff, Jarek Meller, Nicholas J Talbot, Adam D Price, Yuanwei Zang, Maria F. Czyzyk-Krzeska, Birgit Ehmer, Johnson Chu, David R. Plas
Publikováno v:
The Journal of Cell Biology
The work identifies a noncanonical autophagic program mediated by the hominid-specific LC3C paralog functioning downstream from the von Hippel–Lindau tumor suppressor. The program targets for lysosomal degradation postdivision midbodies in a mechan
Publikováno v:
The Journal of cell biology. 220(11)
Atlastin (ATL) GTPases catalyze homotypic membrane fusion of the peripheral endoplasmic reticulum (ER). GTP-hydrolysis–driven conformational changes and membrane tethering are prerequisites for proper membrane fusion. However, the molecular basis f