Zobrazeno 1 - 10
of 24
pro vyhledávání: '"Martin Egli"'
Autor:
Rachana Tomar, Irina G Minko, Pankaj Sharma, Andrew H Kellum, Li Lei, Joel M Harp, T M Iverson, R Stephen Lloyd, Martin Egli, Michael P Stone
Publikováno v:
Nucleic Acids Research. 51:3754-3769
The N-(2-deoxy-d-erythro-pentofuranosyl)-urea DNA lesion forms following hydrolytic fragmentation of cis-5R,6S- and trans-5R,6R-dihydroxy-5,6-dihydrothymidine (thymine glycol, Tg) or from oxidation of 7,8-dihydro-8-oxo-deoxyguanosine (8-oxodG) and su
Autor:
Martin Egli, Muthiah Manoharan
Publikováno v:
Nucleic Acids Research. 51:2529-2573
Eighteen nucleic acid therapeutics have been approved for treatment of various diseases in the last 25 years. Their modes of action include antisense oligonucleotides (ASOs), splice-switching oligonucleotides (SSOs), RNA interference (RNAi) and an RN
Autor:
Krishna Aluri, Swati Gupta, Sarah Leblanc, Martin Egli, Tim Racie, Muthiah Manoharan, Anna Bisbe, Peter Podbevšek, Vasant Jadhav, John Szeto, Christopher R. Brown, Hartmut Jahns, Ivan Zlatev, Dale C. Guenther, Martin Maier, Pawan Kumar, Adam Castoreno, Janez Plavec, Rohan Degaonkar
Publikováno v:
Nucleic Acids Research
In order to achieve efficient therapeutic post-transcriptional gene-silencing mediated by the RNA interference (RNAi) pathway, small interfering RNAs (siRNAs) must be chemically modified. Several supra-RNA structures, with the potential to stabilize
Autor:
Anna Bisbe, Martin Maier, Tuyen Nguyen, Martin Egli, Jennifer L. S. Willoughby, Muthiah Manoharan, Ivan Zlatev, Masaaki Akabane-Nakata, Matt Hettinger, Shigeo Matsuda, Hartmut Jahns, Christopher R. Brown, Nate Taneja, Rajar M. Manoharan, Kallanthottathil G. Rajeev, Klaus Charisse
Publikováno v:
Nucleic Acids Research. 50:1221-1240
A critical challenge for the successful development of RNA interference-based therapeutics therapeutics has been the enhancement of their in vivo metabolic stability. In therapeutically relevant, fully chemically modified small interfering RNAs (siRN
Autor:
Joel M Harp, Terry P Lybrand, Pradeep S Pallan, Leighton Coates, Brendan Sullivan, Martin Egli
Publikováno v:
Nucleic acids research. 50(13)
The ribose 2′-hydroxyl is the key chemical difference between RNA and DNA and primary source of their divergent structural and functional characteristics. Macromolecular X-ray diffraction experiments typically do not reveal the positions of hydroge
Autor:
Mark K Schlegel, Maja M Janas, Yongfeng Jiang, Joseph D Barry, Wendell Davis, Saket Agarwal, Daniel Berman, Christopher R Brown, Adam Castoreno, Sarah LeBlanc, Abigail Liebow, Tara Mayo, Stuart Milstein, Tuyen Nguyen, Svetlana Shulga-Morskaya, Sarah Hyde, Sally Schofield, John Szeto, Lauren Blair Woods, Vedat O Yilmaz, Muthiah Manoharan, Martin Egli, Klaus Charissé, Laura Sepp-Lorenzino, Patrick Haslett, Kevin Fitzgerald, Vasant Jadhav, Martin A Maier
Publikováno v:
Nucleic acids research. 50(12)
Preclinical mechanistic studies have pointed towards RNA interference-mediated off-target effects as a major driver of hepatotoxicity for GalNAc–siRNA conjugates. Here, we demonstrate that a single glycol nucleic acid or 2′–5′-RNA modificatio
Publikováno v:
Nucleic Acids Research
Even in high-quality X-ray crystal structures of oligonucleotides determined at a resolution of 1 Å or higher, the orientations of first-shell water molecules remain unclear. We used cryo neutron crystallography to gain insight into the H-bonding pa
Autor:
Mark K Schlegel, Derek K O'Flaherty, Maja M. Janas, Jason A. Gilbert, Namrata D. Erande, Ivan Zlatev, Shigeo Matsuda, Yongfeng Jiang, June Qin, Pawan Kumar, Rohan Degaonkar, Martha Mendez, Masaaki Akabane-Nakata, Muthiah Manoharan, Martin Egli, Lauren Blair Woods
Publikováno v:
Nucleic Acids Research
We recently reported the synthesis of 2′-fluorinated Northern-methanocarbacyclic (2′-F-NMC) nucleotides, which are based on a bicyclo[3.1.0]hexane scaffold. Here, we analyzed RNAi-mediated gene silencing activity in cell culture and demonstrated
Autor:
Pawan Kumar, Piet Herdewijn, Joel M. Harp, Ivan Zlatev, Muthiah Manoharan, Marie Capobianco, Martin Egli, Mark K Schlegel, Mikhail Abramov, Maja M. Janas, Charalambos Kaittanis, Yongfeng Jiang, Adam Castoreno, Rohan Degaonkar, Dale C. Guenther, Guy Schepers
Publikováno v:
Nucleic Acids Research
In this report, we investigated the hexopyranose chemical modification Altriol Nucleic Acid (ANA) within small interfering RNA (siRNA) duplexes that were otherwise fully modified with the 2'-deoxy-2'-fluoro and 2'-O-methyl pentofuranose chemical modi
Autor:
John Szeto, Adam Castoreno, Muthiah Manoharan, Daniel Berman, Sally Schofield, Klaus Charisse, Christopher R. Brown, Shigeo Matsuda, Joel M. Harp, Mark K Schlegel, Joseph D. Barry, Martin Maier, Martin Egli
Publikováno v:
Nucleic Acids Research
We recently reported that RNAi-mediated off-target effects are important drivers of the hepatotoxicity observed for a subset of GalNAc–siRNA conjugates in rodents, and that these findings could be mitigated by seed-pairing destabilization using a s