Zobrazeno 1 - 4
of 4
pro vyhledávání: '"Ying Zhuo"'
Autor:
Wei Li Thong, Yingxin Zhang, Ying Zhuo, Katherine J. Robins, Joanna K. Fyans, Abigail J. Herbert, Brian J. C. Law, Jason Micklefield
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
Engineering biosynthetic assembly lines is a powerful path to new natural products but is challenging with current methods. Here the authors use CRISPR-Cas9 to exchange subdomains within NRPS to alter substrate selectivity.
Externí odkaz:
https://doaj.org/article/65ce6f9e89b844d7adc627e3f70e0f01
Autor:
Mindong Liang, Zilong Li, Weishan Wang, Jiakun Liu, Leshi Liu, Guoliang Zhu, Loganathan Karthik, Man Wang, Ke-Feng Wang, Zhong Wang, Jing Yu, Yuting Shuai, Jiaming Yu, Lu Zhang, Zhiheng Yang, Chuan Li, Qian Zhang, Tong Shi, Liming Zhou, Feng Xie, Huanqin Dai, Xueting Liu, Jingyu Zhang, Guang Liu, Ying Zhuo, Buchang Zhang, Chenli Liu, Shanshan Li, Xuekui Xia, Yaojun Tong, Yanwen Liu, Gil Alterovitz, Gao-Yi Tan, Li-Xin Zhang
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-9 (2019)
Bacterial allosteric transcription factors can sense and respond to a variety of small molecules. Here the authors present CaT-SMelor which uses Cas12a and allosteric transcription factors to detect small molecules in the nanomolar range.
Externí odkaz:
https://doaj.org/article/4207fa0937a94606979b5c111fae58fa
Autor:
Jason Micklefield, Ying Zhuo, Katherine J Robins, Yingxin Zhang, Joanna K Fyans, Brian J. C. Law, Wei Li Thong, Abigail J Herbert
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
Thong, W L, Zhang, Y, Zhuo, Y, Robins, K J, Fyans, J K, Herbert, A J, Law, B J C & Micklefield, J 2021, ' Gene editing enables rapid engineering of complex antibiotic assembly lines ', Nature Communications, vol. 12, no. 1, 6872 . https://doi.org/10.1038/s41467-021-27139-1
Nature Communications
Thong, W L, Zhang, Y, Zhuo, Y, Robins, K J, Fyans, J K, Herbert, A J, Law, B J C & Micklefield, J 2021, ' Gene editing enables rapid engineering of complex antibiotic assembly lines ', Nature Communications, vol. 12, no. 1, 6872 . https://doi.org/10.1038/s41467-021-27139-1
Nature Communications
Re-engineering biosynthetic assembly lines, including nonribosomal peptide synthetases (NRPS) and related megasynthase enzymes, is a powerful route to new antibiotics and other bioactive natural products that are too complex for chemical synthesis. H
Autor:
Chuan Li, Zhiheng Yang, Guang Liu, Ke-Feng Wang, Weishan Wang, Yaojun Tong, Xuekui Xia, Yanwen Liu, Tong Shi, Gao-Yi Tan, Huanqin Dai, Zhong Wang, Buchang Zhang, Ying Zhuo, Shanshan Li, Jie Zhang, Leshi Liu, Zilong Li, Liming Zhou, Lixin Zhang, Mindong Liang, Qian Zhang, Chenli Liu, Yuting Shuai, Jing Yu, Lu Zhang, Gil Alterovitz, Feng Xie, Jiakun Liu, Man Wang, Jiaming Yu, Xueting Liu, Loganathan Karthik, Guoliang Zhu
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-9 (2019)
Nature Communications
Liang, M, Li, Z, Wang, W, Liu, J, Liu, L, Zhu, G, Karthik, L, Wang, M, Wang, K F, Wang, Z, Yu, J, Shuai, Y, Yu, J, Zhang, L, Yang, Z, Li, C, Zhang, Q, Shi, T, Zhou, L, Xie, F, Dai, H, Liu, X, Zhang, J, Liu, G, Zhuo, Y, Zhang, B, Liu, C, Li, S, Xia, X, Tong, Y, Liu, Y, Alterovitz, G, Tan, G Y & Zhang, L X 2019, ' A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules ', Nature Communications, vol. 10, no. 1, 3672 . https://doi.org/10.1038/s41467-019-11648-1
Nature Communications
Liang, M, Li, Z, Wang, W, Liu, J, Liu, L, Zhu, G, Karthik, L, Wang, M, Wang, K F, Wang, Z, Yu, J, Shuai, Y, Yu, J, Zhang, L, Yang, Z, Li, C, Zhang, Q, Shi, T, Zhou, L, Xie, F, Dai, H, Liu, X, Zhang, J, Liu, G, Zhuo, Y, Zhang, B, Liu, C, Li, S, Xia, X, Tong, Y, Liu, Y, Alterovitz, G, Tan, G Y & Zhang, L X 2019, ' A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules ', Nature Communications, vol. 10, no. 1, 3672 . https://doi.org/10.1038/s41467-019-11648-1
Besides genome editing, CRISPR-Cas12a has recently been used for DNA detection applications with attomolar sensitivity but, to our knowledge, it has not been used for the detection of small molecules. Bacterial allosteric transcription factors (aTFs)