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Publikováno v:
Molecular Endocrinology. 3:1279-1288
Expression of the p85gag-mos oncoprotein in temperature sensitive transformed 6m2 cells results in desensitization of glucocorticoid induction of metallothionein- 1 mRNA. Indirect immunofluorescence analyses demonstrate that hormone insensitivity in
Publikováno v:
Molecular Endocrinology. 3:1270-1278
Progesterone receptor-containing T47D human breast cancer cells are responsive to progestins but fail to respond to other steroid hormones, in particular dexamethasone, because they have no measurable levels of receptors for estrogens, androgens, or
Autor:
James Picado-Leonard, Walter L. Miller
Publikováno v:
Molecular Endocrinology. 2:1145-1150
The amino acid sequences of two steroidogenic enzymes, P450c17 (steroid 17α-hydroxylase/17,20 lyse) and P450c21 (steroid 21-hydroxylase), are only 28.9% identical. However, these proteins share a region of 21 amino acids bearing 17 identical residue
Publikováno v:
Molecular Endocrinology. 1:933-941
The effect of the glucocorticoid dexamethasone on the production and degradation of rat GH (rGH) cytoplasmic mRNA was studied in cultured rat pituitary tumor (GC) cells. The incorporation of [3H]uridine into both rGH cytoplasmic mRNA and the pyrimidi
Autor:
S. Stoney Simons, Gertraud Wasner
Publikováno v:
Molecular Endocrinology. 1:109-120
The two independently derived hepatoma cell lines (HTC and Fu5-5) have previously been shown to display different sensitivities for the induction of tyrosine aminotransferase (TAT) enzyme activity and mRNA levels by glucocorticoids with the enzyme be
Autor:
Frank S. French, Paul S. Rennie, Lisa Main, Helen Cheng, Elizabeth M. Wilson, Nicholas Bruchovsky, Simon N. Freeman, Jean Francois Bowden, Dennis B. Lubahn
Publikováno v:
Molecular Endocrinology. 3:703-708
The ability of high doses of cortisol to retard the involution process in the rat ventral prostate was related to alterations in the pattern of gene expression. Poly(A)+ RNA preparations from the prostates of noncastrated, castrated, and castrated ra
Publikováno v:
Molecular Endocrinology. 3:356-362
Previous analyses have indicated that steroid hormone receptors undergo an allosteric change in structure upon binding by the steroid ligand. This structural change was envisioned as an intramolecular unmasking of the protein's DNA-binding domain, th
Autor:
Sophia Y. Tsai, Jonathan F. Elliston, Dean P. Edwards, Ming-Jer Tsai, Bert W. O'Malley, Milan K. Bagchi
Publikováno v:
Molecular Endocrinology. 2:1221-1229
We investigated the requirement of steroid hormone for the specific binding of progesterone receptor to its cognate progesterone responsive element (PRE) in cell-free experiments. We prepared unfractionated nuclear extracts from human breast cancer (
Publikováno v:
Molecular Endocrinology. 3:1839-1844
The ductus deferens smooth muscle tumor cell line (DDT1-MF-2) is very sensitive to steroids. Treatment with 10 nM testosterone accelerates the growth of DDT1 cells in the absence of serum. Glucocorticoids in the presence or absence of androgens inhib
Publikováno v:
Molecular Endocrinology. 3:1002-1005
A consensus sequence has been proposed as necessary for steroid binding on the basis of sequence homology in steroidogenic enzymes, steroid binding proteins and steroid receptors. We have mapped the limit of sequences important for steroid binding in