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Autor:
Gerald A. LeBlanc, Joseph J. Morrissey, David J. Waxman, Notani Gur, Scott S. Sundseth, Prabha A. Ram, John A. Alberta
Publikováno v:
Molecular Endocrinology. 4:447-454
Oligonucleotide probes that distinguish between two closely related mRNAs encoding steroid 6 beta-hydroxylases of rat P-450 gene family CYP3A were used to individually assess their responsiveness to pituitary hormone regulation. Northern blot analysi
Autor:
Barry J. Goldstein, Alana L. Dudley
Publikováno v:
Molecular Endocrinology. 4:235-244
To investigate whether alterations in the polypeptide structure of the insulin receptor might explain the heterogeneity observed in its properties between species and in different tissues, we obtained the complete primary structure of the rat liver i
Autor:
Inkyu Lee, Min Hu, Li Wang, Jiansheng Huang, Pradip K. Saha, Yong-Deuk Kim, Keun-Gyu Park, David D. Moore, Rohit N. Kulkarni, Arun S. Rajan, Lawrence Chan
Publikováno v:
Molecular Endocrinology. 22:2203-2203
The orphan receptor small heterodimer partner (SHP; NROB2) is a transcriptional repressor that inhibits nuclear receptor signaling in diverse metabolic pathways. Here, we report that SHP(-/-) mice exhibited hypoinsulinemia with age, which was associa
Autor:
Lisa H. Fish, W B Kinlaw, Harold L. Schwartz, Ana Perez-Castillo, Cary N. Mariash, Jack H. Oppenheimer
Publikováno v:
Molecular Endocrinology. 1:609-613
The mRNA of the rat hepatic S14 gene accumulates rapidly after administration of T3 and carbohydrate, making it an excellent model for studies of the effects of dietary and hormonal stimuli at the hepatocellular level. We undertook studies to assess
Publikováno v:
Molecular Endocrinology. 2:528-535
Alternative splicing of insulin-like growth factor I (IGF-I)/somatomedin C mRNAs generates two IGF-I mRNAs coding for IGF-I peptides with different sequences in the E domain of the IGF-I prohormone. These two mRNAs encode alternative E peptides due t
Publikováno v:
Molecular Endocrinology. 3:580-587
The effects of GH and insulin the accumulation of insulin-like growth factor I (IGF-I) RNA transcripts and the secretion of immunoreactive IGF-I protein were studied in rat liver hepatocytes cultured in serum-free medium. GH at concentrations of 10 n
Publikováno v:
Molecular Endocrinology. 1:233-242
The GH dependence of somatomedin-C/insulin-like growth factor I (Sm-C/IGF-I) and insulin like growth factor II (IGF-II) mRNAs was investigated by Northern blot hybridizations of polyadenylated RNAs from liver, pancreas, and brain of normal rats, untr
Autor:
Donald B. Jump, Gerald J. Lepar
Publikováno v:
Molecular Endocrinology. 3:1207-1214
3T3-F442A cells differentiate from preadipocytes to adipocytes when cultured in medium containing fetal calf serum and insulin. We examined the regulation of the S14 gene in 3T3-F442A preadipocytes and adipocytes to determine whether these cells woul
Publikováno v:
Molecular Endocrinology. 2:1077-1086
To obtain information about the functional significance of the structural heterogeneity that has been described for rat insulin-like growth factor I (IGF-I) cDNAs, we hybridized polyadenylated RNAs from rat tissues at different developmental stages w
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