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Publikováno v:
Molecular cell. 81(21)
Transcription progression is governed by multitasking regulators including SPT5, an evolutionarily conserved factor implicated in virtually all transcriptional steps from enhancer activation to termination. Here we utilize a rapid degradation system
Autor:
Henning Urlaub, Seychelle M. Vos, Christian Dienemann, Patrick Cramer, Ying Chen, Momchil Ninov
Publikováno v:
Molecular cell. 81(16)
Summary The super elongation complex (SEC) contains the positive transcription elongation factor b (P-TEFb) and the subcomplex ELL2-EAF1, which stimulates RNA polymerase II (RNA Pol II) elongation. Here, we report the cryoelectron microscopy (cryo-EM
Autor:
Sousa-Luテュs, R., Dujardin, G., Zukher, I., Kimura, Hiroshi, Weldon, C., Carmo-Fonseca, M., Proudfoot, N. J., Nojima, T.
Publikováno v:
Molecular Cell
Summary Mammalian chromatin is the site of both RNA polymerase II (Pol II) transcription and coupled RNA processing. However, molecular details of such co-transcriptional mechanisms remain obscure, partly because of technical limitations in purifying
Autor:
Martin G. Jaeger, Lucas Farnung, Patrick Cramer, Kristina Žumer, Kerstin C. Maier, Petra Rus, Georg E. Winter
Publikováno v:
Molecular cell. 81(15)
Summary Transcription by RNA polymerase II (RNA Pol II) relies on the elongation factors PAF1 complex (PAF), RTF1, and SPT6. Here, we use rapid factor depletion and multi-omics analysis to investigate how these elongation factors influence RNA Pol II
Autor:
Roberto Ballarino, Toyoaki Natsume, Laura Baranello, Rashid Mehmood, Radosław Grochowski, Anika Wiegard, Jan Grosser, Michele Ceribelli, Donald P. Cameron, Anna H. Bizard, Fedor Kouzine, Nicola Crosetto, Vladislav Kuzin, Arne Lindqvist, Ivana Karabogdan, Francesco Valant
Publikováno v:
Wiegard, A, Kuzin, V, Cameron, D P, Grosser, J, Ceribelli, M, Mehmood, R, Ballarino, R, Valant, F, Grochowski, R, Karabogdan, I, Crosetto, N, Lindqvist, A, Bizard, A H, Kouzine, F, Natsume, T & Baranello, L 2021, ' Topoisomerase 1 activity during mitotic transcription favors the transition from mitosis to G1 ', Molecular Cell, vol. 81, no. 24, pp. 5007-5024.e9 . https://doi.org/10.1016/j.molcel.2021.10.015
As cells enter mitosis, chromatin compacts to facilitate chromosome segregation yet remains transcribed. Transcription supercoils DNA to levels that can impede further progression of RNA polymerase II (RNAPII) unless it is removed by DNA topoisomeras
Publikováno v:
Mol Cell
Pre-mRNA processing steps are tightly coordinated with transcription in many organisms. To determine how co-transcriptional splicing is integrated with transcription elongation and 3’ end formation in mammalian cells, we performed long-read sequenc
Autor:
Maria Victoria Neguembor, Chao-ting Wu, Maria Pia Cosma, Chiara Vicario, Pablo Aurelio Gómez-García, Laura Martin, Álvaro Castells-García, Melike Lakadamyali, Francesco Sottile, Davide Carnevali, Jumana AlHaj Abed, Jérôme Solon, Ruben Sebastian-Perez, Alba Granados
Publikováno v:
Molecular cell. 81(15)
The chromatin fiber folds into loops, but the mechanisms controlling loop extrusion are still poorly understood. Using super-resolution microscopy, we visualize that loops in intact nuclei are formed by a scaffold of cohesin complexes from which the
Autor:
Matthew D. Simon, Nicolle A. Rosa-Mercado, Jesse Rinehart, Maria Apostolidi, Joshua T. Zimmer, Joan A. Steitz
Publikováno v:
Mol Cell
Stress-induced readthrough transcription results in the synthesis of downstream-of-gene (DoG)-containing transcripts. The mechanisms underlying DoG formation during cellular stress remain unknown. Nascent transcription profiles during DoG induction i
Autor:
Jérôme O. Rouvière, Søren Lykke-Andersen, Carina Demel, Torben Heick Jensen, Manfred Schmid, Björn Schwalb, Guifen Wu, Kristina Žumer, Patrick Cramer, Ewa Šmidová Molska
Publikováno v:
Lykke-Andersen, S, Žumer, K, Molska, E Š, Rouvière, J O, Wu, G, Demel, C, Schwalb, B, Schmid, M, Cramer, P & Jensen, T H 2021, ' Integrator is a genome-wide attenuator of non-productive transcription ', Molecular Cell, vol. 81, no. 3, pp. 514-529.e6 . https://doi.org/10.1016/j.molcel.2020.12.014
Termination of RNA polymerase II (RNAPII) transcription in metazoans relies largely on the Cleavage and Polyadenylation (CPA) and Integrator (INT) complexes originally found to act at the ends of protein-coding and snRNA genes, respectively. Here we
Autor:
Patrick Cramer, Michael Lidschreiber, Sara Monteiro-Martins, Anna Sawicka, Livia Caizzi, Björn Schwalb, Ying Chen, Kseniia Lysakovskaia, Jana Schmitzová
Publikováno v:
Molecular cell. 81(9)
Transcription by RNA polymerase II (Pol II) is coupled to pre-mRNA splicing, but the underlying mechanisms remain poorly understood. Co-transcriptional splicing requires assembly of a functional spliceosome on nascent pre-mRNA, but whether and how th