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Publikováno v:
Journal of the American Chemical Society. 9/17/2003, Vol. 125 Issue 37, p11241-11248. 8p. 1 Color Photograph, 8 Diagrams, 1 Chart, 2 Graphs.
Autor:
Peter E. Doan, Patricia C. Dos Santos, Hong-In Lee, Mikhail Laryukhin, Brian M. Hoffman, Lance C. Seefeldt, Dennis R. Dean, Robert Y. Igarashi
Publikováno v:
Journal of the American Chemical Society. 126:9563-9569
Nitrogenase is the metalloenzyme that catalyzes the nucleotide-dependent reduction of N(2), as well as reduction of a variety of other triply bonded substrates, including the alkyne, acetylene. Substitution of the alpha-70(Val) residue in the nitroge